Dynamic histone H3 epigenome marking during the intraerythrocytic cycle of Plasmodium falciparum

被引:162
作者
Salcedo-Amaya, Adriana M. [1 ]
van Driel, Marc A. [1 ]
Alako, Blaise T. [1 ]
Trelle, Morten B. [3 ]
van den Elzen, Antonia M. G. [1 ]
Cohen, Adrian M. [1 ]
Janssen-Megens, Eva M. [1 ]
van de Vegte-Bolmer, Marga [2 ]
Selzer, Rebecca R. [4 ]
Iniguez, A. Leonardo [4 ]
Green, Roland D. [4 ]
Sauerwein, Robert W. [2 ]
Jensen, Ole N. [3 ]
Stunnenberg, Hendrik G. [1 ]
机构
[1] Radboud Univ Nijmegen, Dept Mol Biol, Nijmegen Ctr Mol Life Sci, NL-6500 HB Nijmegen, Netherlands
[2] Radboud Univ Nijmegen Med Ctr, Dept Med Microbiol, NL-6500 HB Nijmegen, Netherlands
[3] Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense, Denmark
[4] Roche NimbleGen Inc, Madison, WI 53711 USA
关键词
chromatin; epigenetics; malaria; MUTUALLY EXCLUSIVE EXPRESSION; ANTIGENIC VARIATION; TRANSCRIPTIONAL CONTROL; METHYLATION STATES; VIRULENCE GENES; CELL-CYCLE; MALARIA; ACETYLATION; TRIMETHYLATION; PROMOTERS;
D O I
10.1073/pnas.0902515106
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Epigenome profiling has led to the paradigm that promoters of active genes are decorated with H3K4me3 and H3K9ac marks. To explore the epigenome of Plasmodium falciparum asexual stages, we performed MS analysis of histone modifications and found a general preponderance of H3/H4 acetylation and H3K4me3. ChIP-on-chip profiling of H3, H3K4me3, H3K9me3, and H3K9ac from asynchronous parasites revealed an extensively euchromatic epigenome with heterochromatin restricted to variant surface antigen gene families (VSA) and a number of genes hitherto unlinked to VSA. Remarkably, the vast majority of the genome shows an unexpected pattern of enrichment of H3K4me3 and H3K9ac. Analysis of synchronized parasites revealed significant developmental stage specificity of the epigenome. In rings, H3K4me3 and H3K9ac are homogenous across the genes marking active and inactive genes equally, whereas in schizonts, they are enriched at the 5' end of active genes. This study reveals an unforeseen and unique plasticity in the use of the epigenetic marks and implies the presence of distinct epigenetic pathways in gene silencing/activation throughout the erythrocytic cycle.
引用
收藏
页码:9655 / 9660
页数:6
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