Antisense p53 decreases production of VEGF in follicular thyroid cancer cells

被引：7

作者：

Hassan, I. ^{[1
]}

Wunderlich, A. ^{[1
]}

Slater, E. ^{[1
]}

Hoffmann, S. ^{[1
]}

Celik, I. ^{[1
]}

Zielke, A. ^{[1
]}

机构：

[1] Univ Marburg, Dept Visceral Thorac & Vasc Surg, D-35043 Marburg, Germany

来源：

ENDOCRINE | 2006年 / 29卷 / 03期

关键词：

undifferentiated thyroid cancer; FTC-1; 33; cell line; p53; VEGF; ODN; angiogenesis;

D O I：

10.1385/ENDO:29:3:409

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Inactivating mutations of wild-type p53 (WTp53) tumor suppressor gene are common in anaplastic thyroid cancer (ATC) and are associated with poor prognosis. Mutated p53 (MTp53) has been implicated with angiogenesis. Therefore, the potential of MTp53 knockout by oligodeoxyribonucleotide phosphorothioates (ODNs) to affect VEGF production of undifferentiated thyroid cancer cells with a recessive MTp53 mutation was evaluated. Transient transfection with 20 bp ODNs complementary to portions of exon 10 of p53 and a negative control ODN (HIV-RT) were carried out in FTC-133 cells. In vitro secretion of VEGF protein was quantified by EIA and correlated to cell numbers, which was evaluated by in vitro MTT assay. Transfection of undifferentiated thyroid cancer cells with ODN reduced VEGF secretion of FTC-133 cells following transfection by 34% as compared to the negative control (cells transfected with ODN-HIV; P = 0.03). These results suggest that transient MTp53 knockout with ODNs complementary to p53 nucleotide sequences impair secretion of VEGF in the undifferentiated thyroid cancer cell line FTC-133.

引用

页码：409 / 412

页数：4

共 20 条

[1]

Bauer AJ, 2003, ANN CLIN LAB SCI, V33, P192

[2] Vascular endothelial growth factor monoclonal antibody inhibits growth of anaplastic thyroid cancer xenografts in nude mice [J].

Bauer, AJ ;

Terrell, R ;

Doniparthi, NK ;

Patel, A ;

Tuttle, RM ;

Saji, M ;

Ringel, MD ;

Francis, GL .

THYROID, 2002, 12 (11) :953-961

[3] SELECTIVE CYTOTOXICITY TO HUMAN LEUKEMIC MYELOBLASTS PRODUCED BY OLIGODEOXYRIBONUCLEOTIDE PHOSPHOROTHIOATES COMPLEMENTARY TO P53 NUCLEOTIDE-SEQUENCES [J].

BAYEVER, E ;

HAINES, KM ;

IVERSEN, PL ;

RUDDON, RW ;

PIRRUCCELLO, SJ ;

MOUNTJOY, CP ;

ARNESON, MA ;

SMITH, LJ .

LEUKEMIA & LYMPHOMA, 1994, 12 (3-4) :223-231

[4] CONTROL OF ANGIOGENESIS IN FIBROBLASTS BY P53 REGULATION OF THROMBOSPONDIN-1 [J].

DAMERON, KM ;

VOLPERT, OV ;

TAINSKY, MA ;

BOUCK, N .

SCIENCE, 1994, 265 (5178) :1582-1584

[5] Growth inhibition of pancreatic tumor cells by modified antisense oligodeoxynucleotides [J].

Fiedler, A ;

Knorre, C ;

Franke, Y ;

Henne-Bruns, D ;

Kremer, B ;

Luttges, J ;

Maier, M ;

Gerster, M ;

Bleicher, K ;

Bayer, E ;

Kalthoff, H .

LANGENBECKS ARCHIVES OF SURGERY, 1998, 383 (3-4) :269-275

[6]

FOLKMAN J, 1986, CANCER RES, V46, P467

[7] Thyrotropin (TSH)-Induced production of vascular endothelial growth factor in thyroid cancer cells in vitro:: Evaluation of TSH signal transduction and of angiogenesis-stimulating growth factors [J].

Hoffmann, S ;

Hofbauer, LC ;

Scharrenbach, V ;

Wunderlich, A ;

Hassan, I ;

Lingelbach, S ;

Zielke, A .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (12) :6139-6145

[8] Immunocytochemical detection of p53 in human thyroid carcinomas is associated with mutation and immortalization of cell lines [J].

Jossart, GH ;

Epstein, HD ;

Shaver, JK ;

Weier, HU ;

Greulich, KM ;

Tezelman, S ;

Grossman, RF ;

Siperstein, AE ;

Duh, QY ;

Clark, OH .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (10) :3498-3504

[9] Loss of p53 promotes anaplasia and local invasion in ret/PTC1-induced thyroid carcinomas [J].

La Perle, KMD ;

Jhiang, SM ;

Capen, CC .

AMERICAN JOURNAL OF PATHOLOGY, 2000, 157 (02) :671-677

[10]

Lessin LS, 2000, THYROID CANCER, P337

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