Light-induced release of the cardioprotective peptide angiotensin-(1-9) from thermosensitive liposomes with gold nanoclusters

被引：12

作者：

Bejarano, Julian ^{[1
,2
,3
]}

Rojas, Aldo ^{[1
,2
,3
,4
]}

Ramirez-Sagredo, Andrea ^{[1
,2
,4
]}

Riveros, Ana L. ^{[1
,2
,3
]}

Morales-Zavala, Francisco ^{[1
,2
,3
,4
]}

Flores, Yvo ^{[1
,2
,3
,4
]}

Riquelme, Jaime A. ^{[1
,2
,3
]}

Guzman, Fanny ^{[5
]}

Araya, Eyleen ^{[1
,2
,6
]}

Chiong, Mario ^{[1
,2
,4
]}

Paz Ocaranza, Maria ^{[1
,2
,7
,8
,9
]}

Morales, Javier O. ^{[1
,2
,7
,8
,10
]}

Villamizar Sarmiento, Maria Gabriela ^{[1
,2
,10
]}

Sanchez, Gina ^{[11
]}

Lavandero, Sergio ^{[1
,2
,4
,11
,12
,13
]}

Kogan, Marcelo J. ^{[1
,2
,3
,12
]}

机构：

[1] Univ Chile, Fac Ciencias Quim & Farmaceut, Adv Ctr Chron Dis ACCDiS, Santiago 8380492, Chile

[2] Univ Chile, Fac Med, Santiago 8380492, Chile

[3] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Quim Farmacol & Toxicol, Santiago 8380492, Chile

[4] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Bioquim, Santiago 8380492, Chile

[5] Pontificia Univ Catolica Valparaiso, Nucleo Biotecnol Curauma NBC, Valparaiso, Chile

[6] Univ Andres Bello, Fac Ciencias Exactas, Dept Ciencias Quim, Santiago 8370035, Chile

[7] Univ Chile, Ctr New Drugs Hypertens CENDHY, Santiago 8380492, Chile

[8] Pontificia Univ Catolica Chile, Santiago 8380492, Chile

[9] Pontificia Univ Catolica Chile, Fac Med, Div Enfermedades Cardiovasc, Santiago 8320000, Chile

[10] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Sci & Pharmaceut Technol, Santiago 8380492, Chile

[11] Univ Chile, Fac Med, Inst Ciencias Biomed ICBM, Santiago 8380453, Chile

[12] Corp Ctr Estudios Cientif Enfermedades Cron CECEC, Santiago 7680201, Chile

[13] Univ Texas Southwestern Med Ctr Dallas, Dept Internal Med, Div Cardiol, Dallas, TX 75390 USA

来源：

JOURNAL OF CONTROLLED RELEASE | 2020年 / 328卷

关键词：

Angiotensin-(1-9); Cardioprotection; Cardiovascular diseases; Thermosensitive liposomes; Nanoparticles; GROWTH-FACTOR-I; CONVERTING-ENZYME; DRUG-DELIVERY; CARDIOVASCULAR-DISEASE; TEMPERATURE; ABSORPTION; TRANSITION; PRESSURE; RAT;

D O I：

10.1016/j.jconrel.2020.11.002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoThermAuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 mu M. This angiotensin-(1-9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43.C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNCbased nanosystem was confirmed using an ex vivo Langendorff heart model.

引用

页码：859 / 872

页数：14

共 64 条

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