Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C ε)

被引：7
|
作者：
Jin, F [1 ]
Ji, CN [1 ]
Liu, LF [1 ]
Dai, JF [1 ]
Gu, SH [1 ]
Sun, XF [1 ]
Xie, Y [1 ]
Mao, YM [1 ]
机构：
[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2004年 / 31卷 / 03期
基金：
中国国家自然科学基金;
关键词：
expression pattern; PP2C epsilon;
D O I：
10.1023/B:MOLE.0000043624.96006.eb
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+-dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C epsilon from rat and mouse. Then we term it human PP2C e gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C e is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.
引用
收藏
页码：197 / 202
页数：6
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