Immunolocalization of a human cementoblastoma-conditioned medium-derived protein

被引:33
作者
Arzate, H
Jiménez-García, LF
Alvarez-Pérez, MA
Landa, A
Bar-Kana, I
Pitaru, S
机构
[1] Univ Nacl Autonoma Mexico, Fac Odontol, Lab Biol Celular & Mol, Div Estudios Posgrado & Invest, Mexico City 04510, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
[3] Tel Aviv Univ, Maurice & Gabriela Goldschleger Sch Dent Med, IL-69978 Tel Aviv, Israel
关键词
cementoblasts; cementum protein; periodontium;
D O I
10.1177/154405910208100808
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Little is known about the molecular mechanisms that regulate the cementogenesis process, because specific cementum markers are not yet available. To investigate whether a cementoblastoma-conditioned medium-derived protein (CP) could be useful as a cementum biological marker, we studied its expression and distribution in human periodontal tissues, human periodontal ligament, alveolar bone, and cementoblastoma-derived cells. In human periodontal tissues, immunoreactivity to anti-CP was observed throughout the cementoid phase of acellular and cellular cementum, cementoblasts, cementocytes, cells located in the endosteal spaces of human alveolar bone, and in cells in the periodontal ligament located near the blood vessels. Immunopurified CP promoted cell attachment on human periodontal ligament, alveolar bone-derived cells, and gingival fibroblasts. A monoclonal antibody against bovine cementum attachment protein (CAP) cross-reacted with CP. These findings indicate that CP identifies potential cementoblast progenitor cells, is immunologically related to CAP species, and serves as a biological marker for cementum.
引用
收藏
页码:541 / 546
页数:6
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