Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments

被引：34

作者：

Antanasijevic, Aleksandar ^{[1
]}

Ramirez, Benjamin ^{[1
]}

Caffrey, Michael ^{[1
]}

机构：

[1] Univ Illinois, Dept Biochem & Mol Genet, Chicago, IL 60607 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 2014年 / 60卷 / 01期

关键词：

BSA; Hemagglutinin; Ribosome; STD; WaterLOGSY; LIGAND; BINDING; TARGET; PROTEINS; AFFINITY;

D O I：

10.1007/s10858-014-9848-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The WaterLOGSY (WL) and saturation transfer difference (STD) NMR experiments have proven to be extremely useful techniques to characterize interactions between small molecules and large biomolecules. In this work we compare the relative sensitivities of WL and STD NMR using 3 experimental systems: ketoprofen (KET)-bovine serum albumin (BSA), tert-butyl hydroquinone (TBHQ)-hemagglutinin (HA), and chloramphenicol (CAM)-ribosome (70S). In all cases we find that WL is more sensitive than STD for a given experimental time with the ratios ranging from 3.2 for KET-BSA to 16 for TBHQ-HA and CAM-70S. We attribute the increased sensitivity of WL to be due to simultaneous saturation of multiple sources of cross correlation, including direct NOEs of H-1 of water and exchangeable groups and indirect NOEs of H-1-C groups. We suggest that the outstanding sensitivity of WL make it ideally suited for drug screening efforts targeting very large biomolecules at relatively low concentrations.

引用

页码：37 / 44

页数：8

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