Microplastic Contamination and Ecological Status of Freshwater Ecosystems: A Case Study in Two Northern Portuguese Rivers

Background: Most European rivers have not yet achieved "good" ecological status. In addition, the presence and abundance of microplastics (MPs) in freshwater is a matter of great concern to the scientific community. Methodology: This study assesses the ecological status of four sampling sites of Selho (S1-S4) and Costa-Couros (C1-C4) rivers (Guimaraes, Portugal), and the abundance of MPs in sediments and benthic macroinvertebrates (Oligochaeta) from those sites. Results: All sites of both rivers under higher pressure did not reach a "good" ecological status (S2-S3, C2-C4) based on the macroinvertebrate community. High levels of nutrients were observed at all sites in both rivers (except C2), especially phosphorus. In the Oligochaeta's gut of Costa-Couros river, the high number of MPs seems to be associated with their weight (95.25 +/- 25.61 to 1069.00 +/- 385.30 MPs g/fresh weight), suggesting the existence of malnutrition and digestive disorders, whereas the highest number of MPs in gut (134.00 +/- 30.36 to 558.6 +/- 100.70 MPs g/fresh weight) were found for the heaviest organisms of Selho. Conclusions: Thus, sites with higher ecological status do not necessarily have lower abundance of MPs. In the sediments, urbanization seems to be the main driver for MP contamination. MP contamination is pervasive across the sediments and Oligochaeta's gut in both rivers. Since MPs have the potential to cause harm to environmental and human health, it is essential to monitor not only the ecological status of freshwaters, but also emerging pollutants such as MPs.