Oxidative modification of blood serum proteins in multiple sclerosis after interferon or mitoxantrone treatment

This study was aimed at (i) comparison of the usefulness of serum protein oxidation parameters for assessment of oxidative stress (OS) in multiple sclerosis (MS), and (ii) comparison of OS in MS patients subject to various therapies. Elevated glycophore level was noted in relapsing-remitting (RRMS) patients without treatment and patients treated with interferons beta 1a and beta 1b (10.33 +/- 3.27, 8.02 +/- 2.22 and 8.56 +/- 2.45 vs control 5.27 +/- 0.73 fluorescence units (FU)/mg protein). Advanced oxidation protein products (295 +/- 135 vs 83 +/- 65 nmol/mg protein), carbonyl groups (3.68 +/- 1.44 nmol/mg protein vs 2.03 +/- 0.23 nmol/mg protein), kynurenine (7.71 +/- 0.1.67 vs 5.5 +/- 0.63 FU/mg protein) and N'-formylkynurenine (7.69 +/- 0.7 vs 4.97 +/- 0.59 FU/mg protein) levels were increased, while thioredoxin level was decreased in RRMS patients without treatment (5.03 +/- 2.18 vs 10.83 +/- 2.75 ng/ml) with respect to control. The level of OS was higher in untreated RRMS patients and in SPMS patients treated with mitoxantrone than in patients treated with interferon. (C) 2013 Elsevier BAT. All rights reserved.