Molecular Survey of the Dissemination of Two blaKPC-Harboring IncFIA Plasmids in New Jersey and New York Hospitals

被引:56
作者
Chen, Liang [1 ]
Chavda, Kalyan D. [1 ]
Melano, Roberto G. [2 ,3 ,4 ]
Hong, Tao [5 ]
Rojtman, Albert D. [6 ]
Jacobs, Michael R. [7 ,8 ]
Bonomo, Robert A. [9 ,10 ,11 ,12 ,13 ]
Kreiswirth, Barry N. [1 ]
机构
[1] Rutgers State Univ, Publ Hlth Res Inst Ctr, New Jersey Med Sch, Newark, NJ 07102 USA
[2] Ontario Agcy Hlth Protect & Promot, Toronto, ON, Canada
[3] Univ Toronto, Toronto, ON, Canada
[4] Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada
[5] Hackensack Med Ctr, Dept Pathol, Hackensack, NJ 07604 USA
[6] Jersey Shore Univ, Dept Pathol, Med Ctr, Neptune, NJ USA
[7] Case Western Reserve Univ, Dept Pathol, Cleveland, OH 44106 USA
[8] Univ Hosp Case Med Ctr, Cleveland, OH USA
[9] Louis Stokes Cleveland Dept Vet Affairs Med Ctr, Res Serv, Cleveland, OH USA
[10] Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA
[11] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA
[12] Case Western Reserve Univ, Dept Mol Biol, Cleveland, OH 44106 USA
[13] Case Western Reserve Univ, Dept Microbiol, Cleveland, OH 44106 USA
基金
美国国家卫生研究院;
关键词
COMPLETE NUCLEOTIDE-SEQUENCE; KLEBSIELLA-PNEUMONIAE; BLA(KPC) GENE; ENTEROBACTER-CLOACAE; TN4401; RESISTANCE;
D O I
10.1128/AAC.02749-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains have spread worldwide and become a major threat in health care facilities. Transmission of bla(KPC), the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novel bla(KPC-3)-harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (tra) and the origin of transfer (oriT) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harbors tra and oriT. A PCR scheme was designed to detect the distribution of bla(KPC)-harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinical Enterobacteriaceae isolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491 K. pneumoniae isolates, and all carried bla(KPC-3). pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258) K. pneumoniae clone, while pBK30683-like plasmids were widely distributed in ST258 and other K. pneumoniae sequence types and among non-K. pneumoniae Enterobacteriaceae species. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination of bla(KPC)-harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids.
引用
收藏
页码:2289 / 2294
页数:6
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