An AC electrokinetic impedance immunosensor for rapid detection of tuberculosis

被引:49
作者
Cui, Haochen [1 ]
Li, Shanshan [1 ,2 ]
Yuan, Quan [1 ]
Wadhwa, Ashutosh [3 ]
Eda, Shigetoshi [3 ]
Chambers, Mark [4 ]
Ashford, Roland [4 ]
Jiang, Hongyuan [2 ]
Wu, Jie [1 ]
机构
[1] Univ Tennessee, Dept Elect Engn & Comp Sci, Knoxville, TN 37996 USA
[2] Harbin Inst Technol, Sch Mech Engn, Harbin 150001, Peoples R China
[3] Univ Tennessee, Dept Forestry Fisheries & Wildlife, Knoxville, TN 37996 USA
[4] Anim Hlth & Vet Labs Agcy, Dept Bovine TB, Addlestone KT15 3NB, Surrey, England
基金
美国国家科学基金会;
关键词
CHIP APPLICATIONS; IMMUNOASSAY; BIOSENSORS; DIAGNOSIS; FLUIDS; SPECTROSCOPY; PARTICLE; ARRAYS;
D O I
10.1039/c3an01112g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This work presents an AC electrokinetic impedance sensing method that is capable of detecting specific interactions between macromolecules such as antigen-antibody binding. Serum samples were added to the surface of interdigitated electrodes that had been coated with bacterial antigens. After applying an AC signal of 100 mV at a specific frequency continuously, the electrodes' impedance change was recorded and used to determine the occurrence and level of antibody binding to the antigen. Our theoretical analysis indicated that with this AC signal, the target macromolecules will experience a sufficiently strong attraction force towards the electrode surface for acceleration of the binding process. Using this method, 11 human tuberculosis and 10 bovine tuberculosis serum samples were tested. The results were consistent with those obtained by a conventional ELISA method. The limit of detection of the impedance sensing method was estimated to be better than 10 ng mL(-1). In summary, we demonstrate that AC electrokinetic impedance sensing can be used for rapid and sensitive detection of specific antibodies in serum samples. This method may form a basis for development of a point of care diagnostic device for human and bovine tuberculosis.
引用
收藏
页码:7188 / 7196
页数:9
相关论文
共 34 条
[11]   Development of an AC electrokinetics-based immunoassay system for on-site serodiagnosis of infectious diseases [J].
Liu, Xiaozhu ;
Yang, Kai ;
Wadhwa, Ashutosh ;
Eda, Shigetoshi ;
Li, Shanshan ;
Wu, Jie .
SENSORS AND ACTUATORS A-PHYSICAL, 2011, 171 (02) :406-413
[12]   Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection [J].
Mak, Andy C. ;
Osterfeld, Sebastian J. ;
Yu, Heng ;
Wang, Shan X. ;
Davis, Ronald W. ;
Jejelowo, Olufisayo A. ;
Pourmand, Nader .
BIOSENSORS & BIOELECTRONICS, 2010, 25 (07) :1635-1639
[13]  
Morgan H., 2003, AC ELECTROKINETICS C
[14]   Competitive quenching fluorescence immunoassay for chlorophenols based on laser-induced fluorescence detection in microdroplets [J].
Nichkova, M ;
Feng, J ;
Sanchez-Baeza, F ;
Marco, MP ;
Hammock, BD ;
Kennedy, IM .
ANALYTICAL CHEMISTRY, 2003, 75 (01) :83-90
[15]   Surface plasmon resonance - Applications in understanding receptor-ligand interaction [J].
Pattnaik, P .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2005, 126 (02) :79-92
[16]   Impedimetric measurements for monitoring avidin-biotin interaction on self-assembled monolayer [J].
Pruneanu, Stela ;
Boughriet, A. ;
Henderson, A. ;
Malins, C. ;
Ali, Z. ;
Olenic, Liliana .
PARTICULATE SCIENCE AND TECHNOLOGY, 2008, 26 (02) :136-144
[17]   Electrochemical impedance spectroscopy of polypyrrole based electrochemical immunosensor [J].
Ramanavicius, A. ;
Finkelsteinas, A. ;
Cesiulis, H. ;
Ramanaviciene, A. .
BIOELECTROCHEMISTRY, 2010, 79 (01) :11-16
[18]   Ac electrokinetics: a review of forces in microelectrode structures [J].
Ramos, A ;
Morgan, H ;
Green, NG ;
Castellanos, A .
JOURNAL OF PHYSICS D-APPLIED PHYSICS, 1998, 31 (18) :2338-2353
[19]  
Reverberi Roberto, 2007, Blood Transfus, V5, P227, DOI 10.2450/2007.0047-07
[20]  
S Li, 2014, BIOSENS BIOELECTRON, V51, P437