Characterization of a Trypanosoma cruzi antigen with homology to intracellular mammalian lectins

被引:2
作者
Macedo, C. D. S.
DaRocha, W. D.
Mortara, R. A.
Prado, M. A. M.
Teixeira, S. M. R. [1 ]
机构
[1] Univ Fed Minas Gerais, ICB, Dept Bioquim & Imunol, BR-31270010 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, ICB, Howard Hughes Med Inst, BR-31270010 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Dept Farmacol, Belo Horizonte, MG, Brazil
[4] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP, Brazil
关键词
Trypanosoma cruzi; antigen; lectin; intracellular trafficking; Chagas disease;
D O I
10.1016/j.ijpara.2006.08.010
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Two cDNAs, isolated from a Trypanosoma cruzi amastigote library immunoscreened with sera from patients with Chagas disease, encode proteins with sequence homology to eukaryotic components of the cellular sorting and recycling machinery. These proteins, denominated TcAGL, present an N-terminal lectin domain and a C-terminal region containing repetitive amino acids and a poly-glutamine tract. They are products of polymorphic alleles of a single copy gene constitutively expressed during the parasite life cycle. Polyclonal antibodies obtained from mice immunized with the recombinant antigen recognize proteins with apparent molecular weight ranging from 95 to 120 kDa in cell lysates from all three life stages and in various strains of the parasite. Sera from Chagas disease patients recognize the recombinant antigen in ELISA and immunoprecipitation assays but not in Western blot assays under denaturing conditions. Consistent with its proposed role in the glycoprotein secreting pathway, immunofluorescence analyses and expression of a green fluorescent protein-tagged TcAGL protein indicate a sub-cellular localization in the vicinity of the flagellar pocket membrane and the Golgi complex of the parasite. (c) 2006 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1473 / 1484
页数:12
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