Differential effects of ethanol on glycine uptake mediated by the recombinant GLYT1 and GLYT2 glycine transporters

被引：20

作者：

Núñez, E ^{[1
]}

López-Corcuera, B ^{[1
]}

Martínez-Maza, R ^{[1
]}

Aragón, C ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Ciencias, Ctr Biol Mol Severo Ochoa, Dept Biol Mol, E-28049 Madrid, Spain

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 2000年 / 129卷 / 04期

关键词：

ethanol; glycine; transport; stable expression; human embryonic kidney cells;

D O I：

10.1038/sj.bjp.0703100

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1 The effects of ethanol on the Function of recombinant glycine transporter 1 (GLYT1) and glycine transporter 2 (GLYT2) have been investigated. 2 GLYT1b and GLYT2a isoforms stably expressed in hut-nan embryonic kidney 293 (HEK 293) cells showed a differential behaviour in the presence of ethanol, only the GLYT2a isoform was acutely inhibited. 3 The cut-off (alcohols with four carbons) displayed by the n-alkanols on GLYT2a indicates that a specific binding site for ethanol exists on GLYT2a or on a GLYT2a-interacting protein. 4 The non-competitive inhibition of GLYT2a indicates an allosteric modulation by ethanol of GLYT2a activity. 5 Chronic treatment with ethanol caused differential adaptive responses on the activity and the membrane expression levels of these transporters. The neuronal GLYT2a isoform decreased in activity and surface expression and the mainly glial GLYT1b isoform slightly increased in function and surface density. 6 These changes may be involved in some of the modifications of glycinergic or glutamatergic neurotransmitter systems produced by ethanol intoxication.

引用

页码：802 / 810

页数：9

共 65 条

[1] GENE STRUCTURE AND GLIAL EXPRESSION OF THE GLYCINE TRANSPORTER GLYT1 IN EMBRYONIC AND ADULT RODENTS [J].