Cooperativity between 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase in the lumen of the endoplasmic reticulum

被引：150
作者：
Bánhegyi, G
Benedetti, A
Fulceri, R
Senesi, S
机构：
[1] Univ Siena, Dept Pathophysiol Expt Med & Publ Hlth, I-53100 Siena, Italy
[2] Hungarian Acad Sci, Endoplasm Reticulum Res Grp, H-1444 Budapest, Hungary
[3] Semmelweis Univ, H-1444 Budapest, Hungary
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2004年 / 279卷 / 26期
关键词：
D O I：
10.1074/jbc.M404159200
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The functional coupling of 11beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase was investigated in rat liver microsomal vesicles. The activity of both enzymes was latent in intact vesicles, indicating the intraluminal localization of their active sites. Glucose-6-phosphate, a substrate for hexose-6-phosphate dehydrogenase, stimulated the cortisone reductase activity of 11B-hydroxysteroid dehydrogenase type 1. Inhibition of glucose-6-phosphate uptake by S3483, a specific inhibitor of the microsomal glucose-6-phosphate transporter, decreased this effect. Similarly, cortisone increased the intravesicular accumulation of radioactivity upon the addition of radiolabeled glucose-6-phosphate, indicating the stimulation of hexose-6-phosphate dehydrogenase activity. A correlation was shown between glucose-6-phosphate-dependent cortisone reduction and cortisone-dependent glucose-6-phosphate oxidation. The results demonstrate a close cooperation of the enzymes based on co-localization and the mutual generation of cofactors for each other.
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页码：27017 / 27021
页数：5
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