Effect of silicon dioxide on expression of poly (ADP-Ribose) polymerase mRNA and protein

被引：10

作者：

Gao, Ai ^{[1
]}

Song, Shanshan ^{[1
]}

Wang, Danlin ^{[1
]}

Peng, Wei ^{[1
]}

Tian, Lin ^{[1
]}

机构：

[1] Capital Med Univ, Sch Publ Hlth & Family Med, Beijing 100069, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Silicon dioxide; PARP; gamma-H2AX; RQ-PCR; Immunofluorescence; POLY(ADP-RIBOSE) POLYMERASE; GENE-EXPRESSION; HISTONE H2AX; DNA-DAMAGE; SERINE; 139; REPAIR; ARSENITE; CELLS; PHOSPHORYLATION; CYTOTOXICITY;

D O I：

10.1016/j.cellbi.2009.04.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells treated with concentrations of silicon dioxide up to 400 mu g/ml was determined by real-time fluorogenetic quantitative PCR (RQ-PCR) and immunofluorescence assay, respectively. MTT assay was used to determine cell viability. The results showed that viability at 400 mg/ml silica was significantly decreased but not at lower concentrations. The protein content of gamma-H2AX in silica-treated group was significantly higher than the controls. The PARP mRNA and protein levels were significantly reduced with a dose response manner from the lowest silicon dioxide level. Our findings suggested that silicon dioxide increased the expression of gamma-H2AX and inhibited the expression of PARP mRNA and protein in Hela cells. (c) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：749 / 754

页数：6

共 22 条

[1] Decreased DNA repair gene expression among individuals exposed to arsenic in United States drinking water [J].

Andrew, AS ;

Karagas, MR ;

Hamilton, JW .

INTERNATIONAL JOURNAL OF CANCER, 2003, 104 (03) :263-268

[2]

[Anonymous], 1997, IARC MONOGR EVAL CAR

[3] Potentiation of anti-cancer agent cytotoxicity by the potent poly(ADP-ribose) polymerase inhibitors NU1025 and NU1064 [J].

Bowman, KJ ;

White, A ;

Golding, BT ;

Griffin, RJ ;

Curtin, NJ .

BRITISH JOURNAL OF CANCER, 1998, 78 (10) :1269-1277

[4] Physiology and pathophysiology of poly(ADP-ribosyl)ation [J].

Bürkle, A .

BIOESSAYS, 2001, 23 (09) :795-806

[5] Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions [J].

D'Amours, D ;

Desnoyers, S ;

D'Silva, I ;

Poirier, GG .

BIOCHEMICAL JOURNAL, 1999, 342 :249-268

[6]

Delaney CA, 2000, CLIN CANCER RES, V6, P2860

[7] Diseases caused by silica: mechanisms of injury and disease development [J].

Ding, M ;

Chen, F ;

Shi, XL ;

Yucesoy, B ;

Mossman, B ;

Vallyathan, V .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2002, 2 (2-3) :173-182

[8] INDIRECT VALIDATION OF A RETROSPECTIVE METHOD OF EXPOSURE ASSESSMENT USED IN A NESTED CASE-CONTROL STUDY OF LUNG-CANCER AND SILICA EXPOSURE [J].

DOSEMECI, M ;

MCLAUGHLIN, JK ;

CHEN, JQ ;

HEARL, F ;

MCCAWLEY, M ;

WU, Z ;

CHEN, RG ;

PENG, KL ;

CHEN, AL ;

REXING, SH ;

BLOT, WJ .

OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, 1994, 51 (02) :136-138

[9] Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis [J].

Fubini, B ;

Hubbard, A .

FREE RADICAL BIOLOGY AND MEDICINE, 2003, 34 (12) :1507-1516

[10] An overview of real-time quantitative PCR: Applications to quantify cytokine gene expression [J].

Giulietti, A ;

Overbergh, L ;

Valckx, D ;

Decallonne, B ;

Bouillon, R ;

Mathieu, C .

METHODS, 2001, 25 (04) :386-401

← 1 2 3 →