Effect of silicon dioxide on expression of poly (ADP-Ribose) polymerase mRNA and protein

被引：10
作者：
Gao, Ai [1 ]
Song, Shanshan [1 ]
Wang, Danlin [1 ]
Peng, Wei [1 ]
Tian, Lin [1 ]
机构：
[1] Capital Med Univ, Sch Publ Hlth & Family Med, Beijing 100069, Peoples R China
来源：
CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Silicon dioxide; PARP; gamma-H2AX; RQ-PCR; Immunofluorescence; POLY(ADP-RIBOSE) POLYMERASE; GENE-EXPRESSION; HISTONE H2AX; DNA-DAMAGE; SERINE; 139; REPAIR; ARSENITE; CELLS; PHOSPHORYLATION; CYTOTOXICITY;
D O I：
10.1016/j.cellbi.2009.04.010
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells treated with concentrations of silicon dioxide up to 400 mu g/ml was determined by real-time fluorogenetic quantitative PCR (RQ-PCR) and immunofluorescence assay, respectively. MTT assay was used to determine cell viability. The results showed that viability at 400 mg/ml silica was significantly decreased but not at lower concentrations. The protein content of gamma-H2AX in silica-treated group was significantly higher than the controls. The PARP mRNA and protein levels were significantly reduced with a dose response manner from the lowest silicon dioxide level. Our findings suggested that silicon dioxide increased the expression of gamma-H2AX and inhibited the expression of PARP mRNA and protein in Hela cells. (c) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：749 / 754
页数：6
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