Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

被引：6

作者：

Bhattacharya, Udayan ^{[1
]}

Adak, Shanta ^{[2
]}

Majumder, Niladri Shekhar ^{[1
]}

Bera, Biswajit ^{[3
]}

Giri, Ashok K. ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Mol & Human Genet Div, Kolkata 700032, India

[2] Seth Anandram Jaipuria Coll, Dept Zool, Kolkata, India

[3] Tea Board, Kolkata, India

来源：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2014年 / 14卷

关键词：

Darjeeling tea; Antimutagenic; Anticancer; Apoptosis; GREEN TEA; BLACK TEA; IN-VITRO; EXTRACT; CELLS; POLYPHENOLS; MUTAGENICITY; (-)-EPIGALLOCATECHIN-3-GALLATE; THEARUBIGINS; INHIBITION;

D O I：

10.1186/1472-6882-14-327

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Background: Darjeeling tea, a most popular variety of black tea, though consumed by the people in different parts of world but its beneficial health effects have not been investigated in details. In this study, the antimutagenic and anticancer effect of Darjeeling tea extract (DTE) has been evaluated. Methods: Antimutagenic activity of the DTE was carried out in two different strains of Salmonella typhimurium by AMES test against a known mutagen benzo[a] pyrene (B[a] P) with S9 activation. Moreover, anticlastogenic property of DTE was also measured by micronuclei formation (MN) against B[a] P with S9 activation in human lymphocytes. The anticancer activity of the same was studied on U937 cell line. Here, Human PBMCs were used as the normal cell control to identify selective anticancer activity of the extract against U937 cells. Results: The results showed significant antimutagenic activity on bacterial strains. A significant decrease in MN was also observed in the DTE treated human lymphocyte cultures pretreated with B[a]P when compared with B[a] P treated cultures alone. The study clearly exhibited anticancer activity of the extract on U937 cell line. Further studies also revealed that apoptosis induction is an important mechanism behind the anticancer effect of DTE. Conclusion: Overall, this study indicates that DTE has significant antimutagenic and anticancer activities on bacterial and mammalian cells respectively.

引用

页数：10

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