Pantoea intestinalis sp nov., isolated from the human gut

A novel bacterial strain, 29Y89B(T), was isolated from a faecal sample of a healthy human subject. Cells were Gram-stain-negative, motile, non-spore-forming and rod-shaped. Strain 29Y89BT formed cream-coloured colonies 2 mm in diameter on trypticase soy agar and showed optimum growth at 35 degrees C. Strain 29Y89BT showed highest 16S rRNA gene sequence similarity to Pantoea gaviniae A18/07(T) (98.4 %) followed by Pantoea calida 1400/07(T). (97.2 %). Multi-locus sequence analysis using atpD (ATP synthase beta subunit), gyrB (DNA gyrase), infB (initiation translation factor 2) and rpoB (RNA polymerase beta subunit) genes also supported the result of 16S rRNA gene sequence based phylogeny. Strain 29Y89B(T) showed 62 and 40.7 % DNA-DNA relatedness with P. calida DSM 22759(T) and P. gaviniae DSM 22758(T). Strain 29Y89B(T) contained C-17 : (0) cyclo, C-19 (:) (0) cyclo omega 8c, C-16 (:) (0), C-14 (:) (0) and C-12 (:) (0) as predominant fatty acids. In addition, strain 29Y89B(T) showed physiological and phenotypic differences from its closest relatives P. gaviniae DSM 22758(T) and P. calida DSM 22759(T). The polar lipid profile mainly comprised phospholipids. The DNA G + C content was 59.1 mol%. Thus, based on the findings of the current study, strain 29Y89BT showed clear delineations from its closest relatives P. gaviniae DSM 22758(T) and P. calida DSM 22759(T), and is thus considered to represent a novel species of the genus Pantoea, for which the name Pantoea intestinalis sp. nov. is proposed. The type strain is 29Y89B(T) (=DSM 28113(T)=MCC 2554(T)).