Development and Application of SCAR Marker for the Detection of Papaya Seed Adulteration in Traded Black Pepper Powder

被引：32

作者：

Dhanya, K. ^{[1
]}

Syamkumar, S. ^{[1
]}

Sasikumar, B. ^{[1
]}

机构：

[1] Indian Inst Spices Res, Crop Improvement & Biotechnol Div, Calicut 673012, Kerala, India

来源：

FOOD BIOTECHNOLOGY | 2009年 / 23卷 / 02期

关键词：

adulteration; black pepper; detection; papaya seed; PCR; SCAR marker; PCR; PRODUCTS; WHEAT;

D O I：

10.1080/08905430902873007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study reports the development of a specific, sensitive, and reproducible Sequence Characterized Amplified Region (SCAR) marker to detect papaya seed powder adulteration in traded black pepper powder. A putative RAPD marker (449 bp) specific to papaya seed was identified, cloned, and sequenced to design the SCAR primers. This specific SCAR marker could detect the presence of papaya seed in all the analyzed simulated standards and in one of five branded market samples of black pepper powder tested. The analytical strategy being very simple could be used for large scale screening of powdered black pepper market samples intended for export and domestic uses.

引用

页码：97 / 106

页数：10

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