Kinetics of enzymatic esterification of glycerol and free fatty acids in crude Jatropha oil by immobilized lipase from Rhizomucor miehei

被引：34

作者：

Gofferje, G. ^{[1
]}

Staebler, A. ^{[1
]}

Herfellner, Thomas ^{[1
]}

Schweiggert-Weisz, Ute ^{[1
]}

Floeter, E. ^{[2
]}

机构：

[1] Fraunhofer Inst Proc Engn & Packaging IVV, D-85354 Freising Weihenstephan, Germany

[2] Tech Univ Berlin, Inst Food Technol & Food Chem, D-14195 Berlin, Germany

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2014年 / 107卷

关键词：

Jatropha oil; Immobilized lipase; Esterification; Glycerol; Inhibition; CANDIDA-RUGOSA LIPASE; CATALYZED ESTERIFICATION; OLEIC-ACID; SUBSTRATE; INHIBITION; HYDROLYSIS; PARAMETERS; MECHANISM; HEXANE; ESTER;

D O I：

10.1016/j.molcatb.2014.05.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymatic neutralization is a recent research focus due to an increasing awareness of environmental problems caused by conventional oil refining. This study investigated the kinetics of enzymatic neutralization in crude Jatropha oil utilizing an immobilized lipase from Rhizomucor miehei. Free fatty acids, in particular oleic acid were esterified with glycerol. The reaction seems to follow a multisubstrate Ping Pong mechanism with competitive inhibition by the acyl acceptors (mono-, diacylglycerides and glycerol). Free fatty acid content did not affect lipase activity within the ranges investigated. The kinetic parameters were determined and showed that enzyme affinity is much higher to glycerol than to free fatty acids. These observations were supported by the fact that the optimum glycerol level is rather small while the reaction rate increases with increasing free fatty acid concentration. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：1 / 7

页数：7

共 38 条

[1] Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM [J].

Basri, Mahiran ;

Kassim, Mohd Azlan ;

Mohamad, Rosfarizan ;

Ariff, Arbakariya B. .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2013, 85-86 :214-219

[2]

Becker K., 2008, Lipid Technology, V20, P104, DOI DOI 10.1002/LITE.200800023

[3] New approaches in deacidification of edible oils - a review [J].

Bhosle, BM ;

Subramanian, R .

JOURNAL OF FOOD ENGINEERING, 2005, 69 (04) :481-494

[4]

Blanch H.W., 1997, BIOCH ENG, P354

[5] Kinetic Modeling of Lipase-Catalyzed Esterification Reaction Between Oleic Acid and Trimethylolpropane: A Simplified Model for Multi-substrate Multi-product Ping-Pong Mechanisms [J].

Bornadel, Amin ;

Akerman, Cecilia Orellana ;

Adlercreutz, Patrick ;

Hatti-Kaul, Rajni ;

Borg, Niklas .

BIOTECHNOLOGY PROGRESS, 2013, 29 (06) :1422-1429

[6] Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system obeys a Ping Pong Bi Bi mechanism with competitive inhibition by the alcohol substrate and water [J].

Bousquet-Dubouch, MP ;

Graber, M ;

Sousa, N ;

Lamare, S ;

Legoy, MD .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2001, 1550 (01) :90-99

[7] Kinetic study on the hydrolysis of palm olein using immobilized lipase [J].

Chew, Yin Hoon ;

Chua, Lee Suan ;

Cheng, Kian Kai ;

Sarmidi, Mohamad Roji ;

Aziz, Ramlan Abdul ;

Lee, Chew Tin .

BIOCHEMICAL ENGINEERING JOURNAL, 2008, 39 (03) :516-520

[8] Rice bran lipase catalyzed esterification of palm oil fatty acid distillate and glycerol in organic solvent [J].

Chong, Fui Chin ;

Tey, Beng Ti ;

Dom, Zanariah Mohd ;

Cheong, Kok Hing ;

Satiawihardja, Budiatman ;

Ibrahim, Mohd Nordin ;

Rahman, Russly Abdul ;

Biak, Dayang Radiah Awang ;

Ling, Tau Chuan .

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2007, 12 (03) :250-256

[9]

Chowdary GV, 2005, INDIAN J CHEM B, V44, P2322

[10]

Deutsche Gesellschaft fur Fettforschung. e.V, 2012, DGF EINH UNT FETT FE

← 1 2 3 4 →