Nicotinic receptor-mediated Ca2+ mobilization and catecholamine secretion in chick adrenal chromaffin cells

被引：4
作者：
Teraoka, H
Matsuzawa, R
Maruyama, Y
Hiraga, T
Ohga, A
机构：
来源：
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1996年 / 72卷 / 03期
关键词：
Adrenal chromaffin cells; Ca2+; catecholamine secretion; chick; muscarinic receptor; nicotinic receptor;
D O I：
10.2183/pjab.72.52
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We reinvestigated the involvement of the nicotinic-acetylcholine receptor in catecholamine secretion from chick adrenal chromaffin cells, Nicotine (10(-8)-10(-4)M), as well as methacholine, caused a dose-dependent increase in the cytosolic concentration of Ca2+ and catecholamine secretion from freshly isolated adrenal chromaffin cells. The effects of acetylcholine (10(-4)M) were effectively inhibited by both hexamethonium (10(-4)M) and atropine (10(-5)M), and almost completely blocked by both antagonists. The results suggest the existence of functional nicotinic receptors, which mediate Ca2+ mobilization and catecholamine secretion, in chromaffin cells of chick adrenal glands.
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页码：52 / 55
页数：4
相关论文
共 19 条
[1]   EFFECTS OF COLLAGENASE ON THE RELEASE OF [H-3]-LABELED NORADRENALINE FROM BOVINE CULTURED ADRENAL CHROMAFFIN CELLS [J].
ALMAZAN, G ;
AUNIS, D ;
GARCIA, AG ;
MONTIEL, C ;
NICOLAS, GP ;
SANCHEZGARCIA, P .
BRITISH JOURNAL OF PHARMACOLOGY, 1984, 81 (04) :599-610
[2]   PREFERENTIAL RELEASE OF ADRENALINE FROM ADRENAL MEDULLA BY MUSCARINE AND PILOCARPINE [J].
DOUGLAS, WW ;
POISNER, AM .
NATURE, 1965, 208 (5015) :1102-&
[3]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[4]   CALCIUM DEPENDENCE OF MUSCARINIC RECEPTOR-MEDIATED CATECHOLAMINE SECRETION FROM THE PERFUSED RAT ADRENAL-MEDULLA [J].
HARISH, OE ;
KAO, LS ;
RAFFANIELLO, R ;
WAKADE, AR ;
SCHNEIDER, AS .
JOURNAL OF NEUROCHEMISTRY, 1987, 48 (06) :1730-1735
[5]  
KAO LS, 1986, J BIOL CHEM, V261, P4881
[6]   CHOLINERGIC MUSCARINIC RECEPTORS OF BOVINE ADRENAL-MEDULLA [J].
KAYAALP, SO ;
NEFF, NH .
NEUROPHARMACOLOGY, 1979, 18 (11) :909-911
[7]   EVIDENCE FOR MUSCARINIC RECEPTORS IN ADRENAL MEDULLA OF DOG [J].
KAYAALP, SO ;
TURKER, RK .
BRITISH JOURNAL OF PHARMACOLOGY, 1969, 35 (02) :265-&
[8]   OBSERVATIONS ON THE MUSCARINIC ACTIVATION OF CATECHOLAMINE SECRETION IN THE CHICKEN ADRENAL [J].
KNIGHT, DE ;
BAKER, PF .
NEUROSCIENCE, 1986, 19 (01) :357-366
[9]   STUDIES OF CHICK ADRENAL-MEDULLA IN ORGAN-CULTURE [J].
LEDBETTER, FH ;
KIRSHNER, N .
BIOCHEMICAL PHARMACOLOGY, 1975, 24 (09) :967-974
[10]   CATECHOLAMINE SECRETION BY HAMSTER ADRENAL-CELLS [J].
LIANG, BT ;
PERLMAN, RL .
JOURNAL OF NEUROCHEMISTRY, 1979, 32 (03) :927-933
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