Heterogeneity in surface sensing suggests a division of labor in Pseudomonas aeruginosa populations

被引:94
作者
Armbruster, Catherine R. [1 ]
Lee, Calvin K. [2 ,3 ,4 ]
Parker-Gilham, Jessica [1 ]
de Anda, Jaime [2 ,3 ,4 ]
Xia, Aiguo [5 ]
Zhao, Kun [6 ,7 ]
Murakami, Keiji [8 ]
Tseng, Boo Shan [9 ]
Hoffman, Lucas R. [1 ,10 ]
Jin, Fan [5 ,11 ]
Harwood, Caroline S. [1 ]
Wong, Gerard C. L. [2 ,3 ,4 ]
Parsek, Matthew R. [1 ,12 ]
机构
[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[2] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA USA
[3] Univ Calif Los Angeles, Dept Chem & Biochem, 405 Hilgard Ave, Los Angeles, CA 90024 USA
[4] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA USA
[5] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei, Anhui, Peoples R China
[6] Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin, Peoples R China
[7] Tianjin Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin, Peoples R China
[8] Tokushima Univ, Grad Sch, Inst Biomed Sci, Dept Oral Microbiol, Tokushima, Japan
[9] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA
[10] Univ Washington, Dept Pediat, Seattle, WA 98195 USA
[11] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Synthet Biol, Shenzhen, Peoples R China
[12] South China Agr Univ, Integrat Microbiol Res Ctr, Guangzhou, Guangdong, Peoples R China
基金
中国国家自然科学基金; 美国国家卫生研究院;
关键词
CYCLIC-DI-GMP; BIOFILM FORMATION; GENE-EXPRESSION; PHENOTYPIC HETEROGENEITY; FLEQ; EXOPOLYSACCHARIDE; DIFFERENTIATION; REPRESSOR; REPORTER; BACTERIA;
D O I
10.7554/eLife.45084
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition between planktonic and biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP in response to surface adherence. Current thinking in the field is that once cells attach to a surface, they uniformly respond by producing c-di-GMP. Here, we describe how the Wsp system generates heterogeneity in surface sensing, resulting in two physiologically distinct subpopulations of cells. One subpopulation has elevated c-di-GMP and produces biofilm matrix, serving as the founders of initial microcolonies. The other subpopulation has low c-di-GMP and engages in surface motility, allowing for exploration of the surface. We also show that this heterogeneity strongly correlates to surface behavior for descendent cells. Together, our results suggest that after surface attachment, P. aeruginosa engages in a division of labor that persists across generations, accelerating early biofilm formation and surface exploration.
引用
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页数:29
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