Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection

被引：127

作者：

Moreau, Gregory Brett ^{[1
]}

Burgess, Stacey L. ^{[1
]}

Sturek, Jeffrey M. ^{[2
]}

Donlan, Alexandra N. ^{[3
]}

Petri, William A., Jr. ^{[1
,3
,4
]}

Mann, Barbara J. ^{[1
,3
]}

机构：

[1] Univ Virginia, Dept Med, Div Infect Dis & Int Hlth, Sch Med, Charlottesville, VA 22908 USA

[2] Univ Virginia, Dept Med, Div Pulm & Crit Care Med, Sch Med, Charlottesville, VA 22908 USA

[3] Univ Virginia, Dept Microbiol Immunol & Canc Biol, Sch Med, Charlottesville, VA 22908 USA

[4] Univ Virginia, Dept Pathol, Sch Med, Charlottesville, VA 22908 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2020年 / 103卷 / 03期

关键词：

ACUTE RESPIRATORY SYNDROME;

D O I：

10.4269/ajtmh.20-0762

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Murine models of SARS-CoV-2 infection are critical for elucidating the biological pathways underlying COVID-19. Because human angiotensin-converting enzyme 2 (ACE2) is the receptor for SARS-CoV-2, mice expressing the human ACE2 gene have shown promise as a potential model for COVID-19. Five mice from the transgenic mouse strain K18-hACE2 were intranasally inoculated with SARS-CoV-2 Hong Kong/VM20001061/2020. Mice were followed twice daily for 5 days and scored for weight loss and clinical symptoms. Infected mice did not exhibit any signs of infection until day 4, when no other obvious clinical symptoms other than weight loss were observed. By day 5, all infected mice had lost around 10% of their original body weight but exhibited variable clinical symptoms. All infected mice showed high viral titers in the lungs as well as altered lung histology associated with proteinaceous debris in the alveolar space, interstitial inflammatory cell infiltration, and alveolar septal thickening. Overall, these results show that the K18-hACE2 transgenic background can be used to establish symptomatic SARS-CoV-2 infection and can be a useful mouse model for COVID-19.

引用

页码：1215 / 1219

页数：5

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