Fluorescent Peptide Sensor for Detection of Copper Ions

被引：1

作者：

Li, A. ^{[1
]}

Wang, Yong ^{[1
,2
]}

机构：

[1] Nanchang Univ, Coll Chem, Nanchang 330031, Jiangxi, Peoples R China

[2] Nanchang Univ, Jiangxi Prov Key Lab Modern Analyt Sci, Nanchang 330031, Jiangxi, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2020年 / 41卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Fluorescence sensor; Peptide; Copper(II) ion; ARGININE;

D O I：

10.7503/cjcu20200447

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A fluorescent sensor for copper (II) ions was developed based on the quenching of rhodamine B-labeled peptide by copper (II). The sensing mechanism was studied using resonance light scattering, UV-Vis absorption spectroscopy, fluorescence lifetime, and circular dichroism. The results showed that copper (II) might induced the conformational change of the peptide that make rhodamine B approach each other, resulting in the aggregation-caused quenching effect of copper(II) and the rhodamine B-labeled peptide. Moreover, the experimental results displayed that the sensor possessed both linear ranges of 5x10(-4)-1x10(-2) mu mol/L and 0.1-7 mu mol/L with a detection limit of 0.29 nmol/L. The sensor also had a high selectivity for copper(II), and can be successfully employed for the detection of copper(II) ions in lake water samples.

引用

页码：2736 / 2741

页数：6

共 16 条

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