Application of Congo Red agar for detection of Streptococcus dysgalactiae isolated from diseased fish

被引：13

作者：

Abdelsalam, M. ^{[1
]}

Nakanishi, K. ^{[2
]}

Yonemura, K. ^{[2
]}

Itami, T. ^{[1
]}

Chen, S. C. ^{[3
]}

Yoshida, T. ^{[1
]}

机构：

[1] Miyazaki Univ, Fac Agr, Miyazaki 8892192, Japan

[2] Miyazaki Fisheries Expt Stn, Miyazaki, Japan

[3] Natl Pingtung Univ Sci & Technol, Dept Vet Med, Pingtung, Taiwan

来源：

JOURNAL OF APPLIED ICHTHYOLOGY | 2009年 / 25卷 / 04期

关键词：

LACTOCOCCUS-GARVIEAE; CELL-SURFACE; INIAE; IDENTIFICATION; VIRULENCE; PATHOGEN; SERIOLA;

D O I：

10.1111/j.1439-0426.2009.01226.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

P>The strong clinical similarity between Lancefield group C Streptococcus dysgalactiae subsp. dysgalactiae (GCSD) and Lactococcus garvieae infections, characterized by development of necrotic lesions in the caudal peduncle of infected fish, has hindered differentiation of these two strains, making rapid and accurate diagnosis of diseased fish in fish farms difficult. GCSD from diseased fish were presumptively identified and isolated using Todd-Hewitt agar containing 30 mu g ml(-1) of Congo Red dye (TH-CR). TH-CR agar was also used to detect and presumptively identify the GCSD obtained from artificially or naturally infected fish. Orange GCSD colonies distinct from the L. garvieae colonies were observed on the TH-CR agar; thus, TH-CR agar can be used to detect and identify GCSD isolated from infected fish.

引用

页码：442 / 446

页数：5

共 23 条

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