Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling-Circle Amplification

被引：426
|
作者：
Cheng, Yongqiang [1 ]
Zhang, Xian [1 ]
Li, Zhengping [1 ]
Jiao, Xiaoxia [1 ]
Wang, Yucong [1 ]
Zhang, Yali [1 ]
机构：
[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Med Chem & Mol Diag Minist Educ, Baoding 071002, Hebei Province, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 18期
基金：
中国国家自然科学基金; 美国国家科学基金会;
关键词：
fluorescence determination; microRNA; rolling-circle amplification; PADLOCK PROBES; SIGNAL AMPLIFICATION; AMPLIFIED SYNTHESIS; GENE-EXPRESSION; DNA DETECTION; RNA LIGASE-2; MICROARRAY; LIGATION; QUANTITATION; SPECIFICITY;
D O I：
10.1002/anie.200805665
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rollingcircle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3268 / 3272
页数：5
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