Protective effect of N-acetylcysteine against ischemia/reperfusion injury in rat urinary bladders

被引：7

作者：

Shin, Ju-Hyun ^{[1
]}

Kim, Gun-Hwa ^{[2
,3
,4
]}

Song, Ki-Hak ^{[1
]}

Na, Yong-Gil ^{[1
]}

Sul, Chong-Koo ^{[1
]}

Lim, Jae-Sung ^{[1
]}

机构：

[1] Chungnam Natl Univ Hosp, Sch Med, Dept Urol, Taejon 301721, South Korea

[2] KBSI, Div Life Sci, Taejon 305333, South Korea

[3] KBSI, Pioneer Res Ctr Prot Network Explorat, Taejon 305333, South Korea

[4] UST, Dept Funct Genom, Taejon 305333, South Korea

来源：

CELL BIOCHEMISTRY AND FUNCTION | 2014年 / 32卷 / 01期

关键词：

urinary bladder; ischemia and reperfusion (I; R) injury; N-acetylcysteine (NAC); oxidative stress; contractile response; ISCHEMIA-REPERFUSION INJURY; PARTIAL OUTLET OBSTRUCTION; OXIDATIVE STRESS; ACETAMINOPHEN OVERDOSE; CONTRACTILE RESPONSES; LIPID-PEROXIDATION; ACETYL CYSTEINE; RABBIT BLADDER; RENAL-FUNCTION; INFARCT SIZE;

D O I：

10.1002/cbf.2967

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ischemia/reperfusion (I/R) injury represents an important cause of bladder contractile dysfunction. One of the major causes leading to this dysfunction is thought to be reactive oxygen species formation. In this study, we investigated the potential benefit of N-acetylcysteine (NAC), a potent antioxidant that neutralizes free radicals, in a rat model of urinary bladder injury. NAC treatment rescues the reduction of contractile response to I/R injury in a dose-dependent manner. In addition, all levels of reactive oxygen species, lipid peroxidation, and NADPH-stimulated superoxide production in the I/R operation+NAC (I/R+NAC) group also decreased compared with a marked increase in the I/R operation+saline (I/R+S) group. Moreover, an in situ fluorohistological approach also showed that NAC reduces the generation of intracellular superoxides enlarged by I/R injury. Together, our findings suggest that NAC has a protective effect against the I/R-induced bladder contractile dysfunction via radical scavenging property. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：24 / 30

页数：7

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