MICROPROPAGATION OF JARTORPHA CURCAS L. THROUGH SHOOT TIP EXPLANTS USING DIFFERENT CONCENTRATIONS OF PHYTOHORMONES

A study was conducted to evaluate the effect of phytohormone BAP alone and in combination with IBA or NAA for in-vitro propagation of Jatropha curcas shoot tip explants. MS medium supplemented with various concentrations of phytohormones significantly affected shoot induction. Maximum (93.33%) shoot induction occurred when MS medium was supplemented with 8.88 mu M BAP or 13.32 and 4.92 mu M of BAP and IBA respectively. Similarly, maximum shoot buds per explant (6.7) and shoot length (4.5 cm) were obtained when 13.32 mu M BAP and 4.92 mu M IBA was added to the MS medium. Shoots produced were re-cultured to MS, 1/2 MS media and MS medium supplemented with different concentrations of IBA and were studied for rooting, where the response was highly significant. IBA supplemented to MS medium at 14.76 mu M gave maximum (4.93) roots per culture.