Alpha-mangostin suppresses IL-6 and IL-8 expression in P. gingivalis LPS-stimulated human gingival fibroblasts

被引：30

作者：

Yiemwattana, Ichaya ^{[1
]}

Kaomongkolgit, Ruchadaporn ^{[2
]}

机构：

[1] Naresuan Univ, Fac Dent, Dept Prevent Dent, Phitsanulok 65000, Thailand

[2] Naresuan Univ, Fac Dent, Dept Oral Diag, Phitsanulok 65000, Thailand

来源：

ODONTOLOGY | 2015年 / 103卷 / 03期

关键词：

Alpha-mangostin; HGFs; IL-6/8; PORPHYROMONAS-GINGIVALIS; PERIODONTAL TISSUES; INTERLEUKIN-8; CELLS; INFLAMMATION; XANTHONES; CYTOKINES;

D O I：

10.1007/s10266-014-0160-7

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 mu g/ml P. gingivalis LPS and alpha-mangostin at 1 mu g/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.

引用

页码：348 / 355

页数：8

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