Fast and precise protein tracking using repeated reversible photoactivation

被引：21
作者：
Chudakov, Dmitriy M.
Chepurnykh, Tatyana V.
Belousov, Vsevolod V.
Lukyanov, Sergey
Lukyanov, Konstantin A.
机构：
[1] RAS, Inst Bioorgan Chem, Moscow 117997, Russia
[2] Evrogen JSC, Moscow 117997, Russia
来源：
TRAFFIC | 2006年 / 7卷 / 10期
关键词：
asCP; asFP595; asulCP; Bid; photoactivatable protein; protein movement tracking; reversible photoactivation;
D O I：
10.1111/j.1600-0854.2006.00468.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.
引用
收藏
页码：1304 / 1310
页数：7
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