Cloning and expression of a candidate malarial epitope in bacille Calmette Guerin

被引：7
作者：
Norazmi, MN
Dale, JW
机构：
[1] UNIV SURREY,SCH BIOL SCI,MOL MICROBIOL GRP,GUILDFORD GU2 5XH,SURREY,ENGLAND
[2] UNIV SAINS MALAYSIA,SCH MED SCI,DEPT IMMUNOL,KUBANG KERAIN,MALAYSIA
来源：
BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 11期
关键词：
D O I：
10.1023/A:1018405013737
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The candidate malarial vaccine epitope, region II of Plasmodium falciparum circumsporozoite protein (RII-CSP), was cloned into Mycobacterium smegmatis and M. bovis BCG via a mycobacterial replicative plasmid pUS1762. This plasmid contained a gene for the M. leprae 18 kDa protein to provide expression signals. Transformation was achieved by electroporation and selection for kanamycin resistance and verified by Southern hybridisation and sequencing. The transformation efficiency was in the order of 10(4) cfu/mg DNA for M. smegmatis and 10(3) cfu/mg DNA for M. bovis BCG. Western blotting using a polyclonal antibody specific for the RII-CSP and a monoclonal antibody specific for the M. leprae protein showed the expected 25 kDa band of the 18 kDa-RII-CSP fusion protein.
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页码：1135 / 1137
页数：3
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