ARID1A is downregulated in non-small cell lung cancer and regulates cell proliferation and apoptosis

被引：51

作者：

Zhang, Yi ^{[1
]}

Xu, Xiaoman ^{[2
]}

Zhang, Meng ^{[1
]}

Bai, Xue ^{[1
]}

Li, Hui ^{[1
]}

Kan, Liang ^{[1
]}

Niu, Huiyan ^{[1
]}

He, Ping ^{[1
]}

机构：

[1] China Med Univ, Dept Geriatr, Shengjing Hosp, Shenyang 110004, Peoples R China

[2] China Med Univ, Dept Resp Med, Shengjing Hosp, Shenyang 110004, Peoples R China

来源：

TUMOR BIOLOGY | 2014年 / 35卷 / 06期

基金：

中国国家自然科学基金;

关键词：

ARID1A; Non-small cell lung cancer; Proliferation; Apoptosis; CHROMATIN-REMODELING GENES; FREQUENT MUTATIONS; SOMATIC MUTATIONS; TUMOR-SUPPRESSOR; GASTRIC-CANCER; CARCINOMA; EXPRESSION; P270; ADENOCARCINOMA; COMPLEXES;

D O I：

10.1007/s13277-014-1755-x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ARID1A (AT-rich interactive domain 1A) is a key member of the SWI/SNF chromatin-modeling complex, and the gene has emerged as a tumor suppressor in various human cancers. In the present study, we investigated the expression and clinical significance of ARID1A in non-small cell lung cancer (NSCLC). We found that ARID1A expression was decreased in NSCLC tissues compared with normal bronchial epithelium and was significantly correlated with nodal metastasis, tumor, node, metastasis (TNM) stage, and poor differentiation. ARID1A expression was lower in lung cancer cell lines than normal bronchial epithelial HBE cell line. We also explored the involvement of ARID1A in biological behavior of lung cancer cell lines. ARID1A depletion by small interfering RNA (siRNA) in H460 and H1299 cell lines promoted proliferation, colony formation ability, and inhibited paclitaxel-induced apoptosis. Furthermore, we identified that ARID1A regulated several cell cycle and apoptosis-related targets such as cyclin D1 and Bcl-2. In addition, the activity of Akt phosphorylation was also enhanced after ARID1A depletion. In conclusion, our data suggested that ARID1A may serve as an important tumor suppressor in NSCLC.

引用

页码：5701 / 5707

页数：7

共 41 条

[1] ARID1A expression loss in gastric cancer: pathway-dependent roles with and without Epstein-Barr virus infection and microsatellite instability [J].

Abe, Hiroyuki ;

Maeda, Daichi ;

Hino, Rumi ;

Otake, Yuya ;

Isogai, Maya ;

Ushiku, Aya Shinozaki ;

Matsusaka, Keisuke ;

Kunita, Akiko ;

Ushiku, Tetsuo ;

Uozaki, Hiroshi ;

Tateishi, Yoko ;

Hishima, Tsunekazu ;

Iwasaki, Yoshiaki ;

Ishikawa, Shumpei ;

Fukayama, Masashi .

VIRCHOWS ARCHIV, 2012, 461 (04) :367-377

[2] Comparative Genomic Analysis of Esophageal Adenocarcinoma and Squamous Cell Carcinoma [J].

Agrawal, Nishant ;

Jiao, Yuchen ;

Bettegowda, Chetan ;

Hutfless, Susan M. ;

Wang, Yuxuan ;

David, Stefan ;

Cheng, Yulan ;

Twaddell, William S. ;

Latt, Nyan L. ;

Shin, Eun J. ;

Wang, Li-Dong ;

Wang, Liang ;

Yang, Wancai ;

Velculescu, Victor E. ;

Vogelstein, Bert ;

Papadopoulos, Nickolas ;

Kinzler, Kenneth W. ;

Meltzer, Stephen J. .

CANCER DISCOVERY, 2012, 2 (10) :899-905

[3]

[Anonymous], 2013, Head Neck Oncol.

[4] Mutations and deletions of ARID1A in breast tumors [J].

Cornen, S. ;

Adelaide, J. ;

Bertucci, F. ;

Finetti, P. ;

Guille, A. ;

Birnbaum, D. J. ;

Birnbaum, D. ;

Chaffanet, M. .

ONCOGENE, 2012, 31 (38) :4255-4256

[5] The human SWI-SNF complex protein p270 is an ARID family member with non-sequence-specific DNA binding activity [J].

Dallas, PB ;

Pacchione, S ;

Wilsker, D ;

Bowrin, V ;

Kobayashi, R ;

Moran, E .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (09) :3137-3146

[6]

Daniel Jonathan C, 2004, Semin Thorac Cardiovasc Surg, V16, P19, DOI 10.1053/j.semtcvs.2004.01.002

[7] Whole-genome sequencing of liver cancers identifies etiological influences on mutation patterns and recurrent mutations in chromatin regulators [J].

Fujimoto, Akihiro ;

Totoki, Yasushi ;

Abe, Tetsuo ;

Boroevich, Keith A. ;

Hosoda, Fumie ;

Nguyen, Ha Hai ;

Aoki, Masayuki ;

Hosono, Naoya ;

Kubo, Michiaki ;

Miya, Fuyuki ;

Arai, Yasuhito ;

Takahashi, Hiroyuki ;

Shirakihara, Takuya ;

Nagasaki, Masao ;

Shibuya, Tetsuo ;

Nakano, Kaoru ;

Watanabe-Makino, Kumiko ;

Tanaka, Hiroko ;

Nakamura, Hiromi ;

Kusuda, Jun ;

Ojima, Hidenori ;

Shimada, Kazuaki ;

Okusaka, Takuji ;

Ueno, Masaki ;

Shigekawa, Yoshinobu ;

Kawakami, Yoshiiku ;

Arihiro, Koji ;

Ohdan, Hideki ;

Gotoh, Kunihito ;

Ishikawa, Osamu ;

Ariizumi, Shun-ichi ;

Yamamoto, Masakazu ;

Yamada, Terumasa ;

Chayama, Kazuaki ;

Kosuge, Tomoo ;

Yamaue, Hiroki ;

Kamatani, Naoyuki ;

Miyano, Satoru ;

Nakagama, Hitoshi ;

Nakamura, Yusuke ;

Tsunoda, Tatsuhiko ;

Shibata, Tatsuhiro ;

Nakagawa, Hidewaki .

NATURE GENETICS, 2012, 44 (07) :760-U182

[8] ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a [J].

Gao, Xiaolin ;

Tate, Peri ;

Hu, Ping ;

Tjian, Robert ;

Skarnes, William C. ;

Wang, Zhong .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (18) :6656-6661

[9] Targeted genomic sequencing of pediatric Burkitt lymphoma identifies recurrent alterations in antiapoptotic and chromatin-remodeling genes [J].

Giulino-Roth, Lisa ;

Wang, Kai ;

MacDonald, Theresa Y. ;

Mathew, Susan ;

Tam, Yifang ;

Cronin, Maureen T. ;

Palmer, Gary ;

Lucena-Silva, Norma ;

Pedrosa, Francisco ;

Pedrosa, Marcia ;

Teruya-Feldstein, Julie ;

Bhagat, Govind ;

Alobeid, Bachir ;

Leoncini, Lorenzo ;

Bellan, Cristiana ;

Rogena, Emily ;

Pinkney, Kerice A. ;

Rubin, Mark A. ;

Ribeiro, Raul C. ;

Yelensky, Roman ;

Tam, Wayne ;

Stephens, Philip J. ;

Cesarman, Ethel .

BLOOD, 2012, 120 (26) :5181-5184

[10] ARID1A, a Factor That Promotes Formation of SWI/SNF-Mediated Chromatin Remodeling, Is a Tumor Suppressor in Gynecologic Cancers [J].

Guan, Bin ;

Wang, Tian-Li ;

Shih, Ie-Ming .

CANCER RESEARCH, 2011, 71 (21) :6718-6727

← 1 2 3 4 5 →