Development of a Novel Chemiluminescence Analysis Using Liquid-Liquid Interface Micro-Reaction Space in a Micro-Channel

A novel chemiluminescence analysis to taked advantage of a specific chemiluminescence reaction space around the liquid-liquid interface in a micro-channel under laminar flow conditions has been reported since 2004. We call the analytical system "micro-channel chemiluminescence analysis (MCCLA)". First, the concept of micro-channel chemiluminescence analysis and its features were described and considered through direct observations of fluorescence and chemiluminescence using a fluorescence microscope-CCD camera and a microscope CCD camera. Next, several concrete examples of micro-channel chemiluminescence analysis were introduced, and the experimental data were basically discussed. A fluorescence compound was examined by micro-channel chemiluminescence analysis using an oxalate chemiluminescence reaction. Anti-oxygen, beverages, and saliva were also investigated by chemiluminescence analysis using a singlet oxygen chemiluminescence reaction. Furthermore, the copper(II) catalyst was determined by chemiluminescence analysis using a luminol reaction. In addition we briefly described feature planes concerning the micro-channel chemiluminescence analysis.