The modulatory effect of infusions of green tea, oolong tea, and black tea on gut microbiota in high-fat-induced obese mice

被引:173
作者
Liu, Zhibin [1 ]
Chen, Zhichao [1 ]
Guo, Hongwen [1 ]
He, Dongping [1 ]
Zhao, Huiru [1 ]
Wang, Zhiyao [1 ]
Zhang, Wen [1 ]
Liao, Lan [1 ]
Zhang, Chen [1 ]
Ni, Li [1 ]
机构
[1] Fuzhou Univ, Inst Food Sci & Technol, Fuzhou 350108, Peoples R China
基金
中国国家自然科学基金;
关键词
DIET; INFLAMMATION; BACTEROIDES; SEQUENCES; CATECHINS; COLITIS; FAMILY; LEAVES;
D O I
10.1039/c6fo01439a
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tea consumption has been identified to have an anti-obesity effect. Whether it is associated with gut microbiota modulation is investigated in this study. Phenolic profiles of infusions of green tea, oolong tea and black tea were comprehensively compared first, by utilizing ultra-performance liquid chromatography- electrospray ionization-quadrupole time-of-flight mass spectrometry ( UPLC-ESI-Q-TOFMS). Subsequently, high-fat-diet induced obese C57BL/6J mice were orally administered these three types of tea infusions for 13 weeks to evaluate their anti-obesity and gut microbiota modulatory effects. In general, 8 phenolic acids, 12 flavanols, 9 flavonols, 2 alkaloids and 1 amino acid were identified from the three types of tea infusions. Though they possess diverse phenolic compounds, no significant differences in the prevention of the development of obesity in high-fat-fed mice were discovered among the three types of tea. Based on high-throughput MiSeq sequencing and multivariate statistical analysis, it was revealed that tea infusion consumption substantially increased diversity and altered the structure of gut microbiota. The linear discriminant analysis effect size algorithm identified 30 key phylotypes in response to high-fat diet and tea, including Alistipes, Rikenella, Lachnospiraceae, Akkermansia, Bacteroides, Allobaculum, Parabacteroides, etc. Moreover, Spearman's correlation analysis indicated that these key phylotypes might have a close association with the obesity related indexes of the host. This study provides detailed information regarding the impact of tea consumption on gut microbiota, which may be helpful in understanding the anti-obesity mechanisms of tea.
引用
收藏
页码:4869 / 4879
页数:11
相关论文
共 53 条
[11]   QIIME allows analysis of high-throughput community sequencing data [J].
Caporaso, J. Gregory ;
Kuczynski, Justin ;
Stombaugh, Jesse ;
Bittinger, Kyle ;
Bushman, Frederic D. ;
Costello, Elizabeth K. ;
Fierer, Noah ;
Pena, Antonio Gonzalez ;
Goodrich, Julia K. ;
Gordon, Jeffrey I. ;
Huttley, Gavin A. ;
Kelley, Scott T. ;
Knights, Dan ;
Koenig, Jeremy E. ;
Ley, Ruth E. ;
Lozupone, Catherine A. ;
McDonald, Daniel ;
Muegge, Brian D. ;
Pirrung, Meg ;
Reeder, Jens ;
Sevinsky, Joel R. ;
Tumbaugh, Peter J. ;
Walters, William A. ;
Widmann, Jeremy ;
Yatsunenko, Tanya ;
Zaneveld, Jesse ;
Knight, Rob .
NATURE METHODS, 2010, 7 (05) :335-336
[12]   Antioxidant activity of white, green and black tea obtained from the same tea cultivar [J].
Carloni, Patricia ;
Tiano, Luca ;
Padella, Lucia ;
Bacchetti, Tiziana ;
Customu, Chisomo ;
Kay, Alexander ;
Damiani, Elisabetta .
FOOD RESEARCH INTERNATIONAL, 2013, 53 (02) :900-908
[13]   The Microbiota Is Essential for the Generation of Black Tea Theaflavins-Derived Metabolites [J].
Chen, Huadong ;
Hayek, Saeed ;
Guzman, Javier Rivera ;
Gillitt, Nicholas D. ;
Ibrahim, Salam A. ;
Jobin, Christian ;
Sang, Shengmin .
PLOS ONE, 2012, 7 (12)
[14]   Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 [J].
Chen, Weihsu C. ;
Kawasaki, Norihito ;
Nycholat, Corwin M. ;
Han, Shoufa ;
Pilotte, Julie ;
Crocker, Paul R. ;
Paulson, James C. .
PLOS ONE, 2012, 7 (06)
[15]   Effects of Green Tea Polyphenol (-)-Epigallocatechin-3-gallate on Newly Developed High-Fat/Western-Style Diet-Induced Obesity and Metabolic Syndrome in Mice [J].
Chen, Yu-Kuo ;
Cheung, Connie ;
Reuhl, Kenneth R. ;
Liu, Anna Ba ;
Lee, Mao-Jung ;
Lu, Yao-Ping ;
Yang, Chung S. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (21) :11862-11871
[16]   Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB [J].
DeSantis, T. Z. ;
Hugenholtz, P. ;
Larsen, N. ;
Rojas, M. ;
Brodie, E. L. ;
Keller, K. ;
Huber, T. ;
Dalevi, D. ;
Hu, P. ;
Andersen, G. L. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (07) :5069-5072
[17]   Identification and comparison of phenolic compounds in the preparation of oolong tea manufactured by semifermentation and drying processes [J].
Dou, Jianpeng ;
Lee, Viola S. Y. ;
Tzen, Jason T. C. ;
Lee, Maw-Rong .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (18) :7462-7468
[18]   Human colonic microbiota associated with diet, obesity and weight loss [J].
Duncan, S. H. ;
Lobley, G. E. ;
Holtrop, G. ;
Ince, J. ;
Johnstone, A. M. ;
Louis, P. ;
Flint, H. J. .
INTERNATIONAL JOURNAL OF OBESITY, 2008, 32 (11) :1720-1724
[19]  
Edgar RC, 2013, NAT METHODS, V10, P996, DOI [10.1038/NMETH.2604, 10.1038/nmeth.2604]
[20]   Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity [J].
Everard, Amandine ;
Belzer, Clara ;
Geurts, Lucie ;
Ouwerkerk, Janneke P. ;
Druart, Celine ;
Bindels, Laure B. ;
Guiot, Yves ;
Derrien, Muriel ;
Muccioli, Giulio G. ;
Delzenne, Nathalie M. ;
de Vos, Willem M. ;
Cani, Patrice D. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (22) :9066-9071