Pulsed Nano-Electrospray Ionization: Characterization of Temporal Response and Implementation with a Flared Inlet Capillary

被引：32

作者：

Bushey, Jared M. ^{[1
]}

Kaplan, Desmond A. ^{[1
]}

Danell, Ryan M. ^{[1
]}

Glish, Gary L. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Venable Lab, Chapel Hill, NC 27599 USA

来源：

INSTRUMENTATION SCIENCE & TECHNOLOGY | 2009年 / 37卷 / 03期

关键词：

Dual ion source; ESI temporal response; Flared inlet capillary; Mass spectrometry; Nano-ESI; Pulsed ESI; QUADRUPOLE ION-TRAP; HIGH-SENSITIVITY ANALYSIS; MASS-SPECTROMETRY; ION/ION REACTIONS; FLOW-RATE; PEPTIDES; NANOELECTROSPRAY; IDENTIFICATION; BIOMOLECULES; ANALYZERS;

D O I：

10.1080/10739140902831313

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The temporal response of pulsed nano-electrospray ionization mass spectrometry (nano-ESI-MS) was studied and its influence on ion formation and detection was characterized. Rise and decay times for the mass resolved ion current were determined to be 203 msec and 615 msec, respectively, which led to a maximum pulse rate of 12Hz. Pulsed nano-ESI operation was demonstrated from a multi-sprayer source controlled by a high voltage pulsing circuit constructed in-house. The desired source mode of operation (e.g. pulsing or continuous) can be realized solely by controlling the voltage applied to each sprayer.

引用

页码：257 / 273

页数：17

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