Clinical significance of kallikrein-related peptidase 7 (KLK7) in colorectal cancer

被引：38
作者：
Talieri, Maroulio [1 ]
Mathioudaki, Konstantina [1 ]
Prezas, Panagiotis [1 ]
Alexopoulou, Dimitra K. [1 ]
Diamandis, Eleftherios P. [2 ]
Xynopoulos, Dimitris [3 ]
Ardavanis, Alexandros [4 ]
Arnogiannaki, Niki [5 ]
Scorilas, Andreas [6 ]
机构：
[1] St Savas Hosp, Dept Cellular Physiol, G Papanicolaou Res Ctr Oncol, Athens 11522, Greece
[2] Mt Sinai Hosp, Dept Pathol & Lab Med, Toronto, ON M5G 1X5, Canada
[3] St Savas Hosp, Dept Gastroenterol, Athens 11522, Greece
[4] St Savas Hosp, Dept Oncol, Athens 11522, Greece
[5] St Savas Hosp, Dept Pathol, Athens 11522, Greece
[6] Univ Athens, Fac Biol, Dept Biochem & Mol Biol, Athens, Greece
来源：
THROMBOSIS AND HAEMOSTASIS | 2009年 / 101卷 / 04期
关键词：
Cell lines; colorectal cancer; human stratum corneum chymotryptic enzyme; KLK7; tumor markers; HUMAN TISSUE KALLIKREINS; CORNEUM CHYMOTRYPTIC ENZYME; MESSENGER-RNA EXPRESSION; APOPTOSIS-RELATED GENES; STRATUM-CORNEUM; OVARIAN-CANCER; PROGNOSTIC-SIGNIFICANCE; BREAST-TUMORS; PROTEOLYTIC CASCADE; LUNG-CARCINOMA;
D O I：
10.1160/TH08-07-0471
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Human tissue kallikrein-related peptidases are a family of 15 secreted serine proteases, located at chromosome 19q 13.4. Most of them have been reported to be potential biomarkers for several carcinomas and other diseases. Human tissue kallikrein-related peptidase 7 (KLK7) has been purified from human stratum corneum and resembles a chymotryptic endopeptidase originally called stratum corneum chymotryptic enzyme (SCCE). In this study, we examined for the first time, the prognostic value of KLK7 mRNA expression, using a semi-quantitative RT-PCR method, in 105 colorectal cancer tissues for 54 of which, paired normal colonic mucosa were available. Furthermore, we analysed the expression of KLK7 in 10 adenomas, in 18 biopsies of inflamed colon mucosa, as well as in 22 human cancer cell lines of various origin, four of them being of colon. A defined number of colon cancer samples were also examined by immunohistochemistry. KLK7 expression was higher in cancerous than in normal tissues. Less differentiated tumors of more advanced stage showed higher KLK7 expression. Follow-up analysis revealed that KLK7 was significantly associated with shorter overall survival (OS) and disease-free survival (DFS). In addition, selected colon cancer samples highly expressing KLK7 gene, showed intense immunohistochemical staining for KLK7, enhancing RTPCR results. Present data suggest that KLK7 gene is up-regulated in colon cancer and its expression predicts poor prognosis for colon cancer patients.
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页码：741 / 747
页数：7
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