Laser Capture Microdissection of Feline Streptomyces spp Pyogranulomatous Dermatitis and Cellulitis

被引：5

作者：

Traslavina, R. P. ^{[1
,2
]}

Reilly, C. M. ^{[1
]}

Vasireddy, R. ^{[3
]}

Samitz, E. M. ^{[1
]}

Stepnik, C. T. ^{[1
]}

Outerbridge, C. ^{[1
]}

Affolter, V. K. ^{[1
]}

Byrne, B. A. ^{[1
]}

Lowenstine, L. J. ^{[1
]}

White, S. D. ^{[1
]}

Murphy, B. ^{[1
]}

机构：

[1] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[2] NINDS, NIH, Sect Infect Nervous Syst, Bethesda, MD 20892 USA

[3] Univ Texas Tyler, Hlth Sci Ctr, Tyler, TX 75799 USA

来源：

VETERINARY PATHOLOGY | 2015年 / 52卷 / 06期

关键词：

cats; Streptomyces; Actinomycetales; dermatitis; cellulitis; laser capture microdissection; mycetoma; ACTINOMYCOTIC MYCETOMA;

D O I：

10.1177/0300985814561094

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Suspected Streptomyces spp infections were identified in 4 cats at UC Davis Veterinary Medical Teaching Hospital between 1982 and 2011. Three had ulcerated, dark red mycetomas involving the dermis, subcutis, and fascia with fistulous tracts and/or regional lymphadenopathy. One cat had pyogranulomatous mesenteric lymphadenitis. Granulomatous inflammation in all cats contained colonies of Gram-positive, non-acid-fast organisms. All 4 cats failed to respond to aggressive medical and surgical treatment and were euthanized. Laser capture microdissection (LCM) was used to selectively harvest DNA from the affected formalin-fixed, paraffin-embedded (FFPE) tissues. Cloned amplicons from LCM-derived tissue confirmed the presence of Streptomyces spp in the dermatitis cases. Amplicons from the remaining cat with peritoneal involvement aligned with the 16S ribosomal RNA gene for Actinomycetales. Usually considered a contaminant, Streptomyces spp can be associated with refractory pyogranulomatous dermatitis and cellulitis in cats with outdoor access. LCM is useful in the diagnosis of bacterial diseases where contamination may be an issue.

引用

页码：1172 / 1175

页数：4

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