Artificial sRNAs activating the Gac/Rsm signal transduction pathway in Pseudomonas fluorescens

被引:7
作者
Valverde, Claudio [1 ]
机构
[1] Univ Nacl Quilmes, Dept Ciencia & Tecnol, Programa Interacc Biol, Buenos Aires, DF, Argentina
关键词
Pseudomonas fluorescens; Riboregulation; Molecular mimicry; Gac/Rsm cascade; Artificial sRNAs; BINDING PROTEIN CSRA; ESCHERICHIA-COLI; REGULATORY RNA; EXTRACELLULAR PROTEASE; EXOPRODUCT FORMATION; GENE-EXPRESSION; MOLECULE CSRB; CHA0; BIOCONTROL; TRANSLATION;
D O I
10.1007/s00203-009-0459-x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In Pseudomonas fluorescens CHA0, the synthesis of antifungal compounds is post-transcriptionally activated by the Gac/Rsm cascade. The two-component system GacS/GacA promotes transcription of three small regulatory RNAs (i.e., sRNAs), RsmX, RsmY, and RsmZ, which remove the regulatory proteins RsmA and RsmE from the ribosome-binding sites of exoproduct-related mRNAs. The GacS/GacA-dependent accumulation of RsmX/Y/Z and formation of RsmX/Y/Z-RsmA/E complexes relieve mRNA translational repression. Other bacteria as E. coli and Vibrio spp. utilize similar sRNA-protein based systems to adjust mRNA translation (e.g., the E. coli Csr system for carbon storage, motility and biofilm regulation). The Rsm/Csr sRNAs are remarkably similar in that they contain several stem-loops with an invariant GGA trinucleotide exposed in the hairpin loop that would be the characteristic structural-sequence motifs relevant for sRNA activity and stability. Here it is shown that the dysfunctional Gac/Rsm cascade of P. fluorescens Delta rsmXYZ mutants could be restored by appropriate transcription levels of artificial genes encoding RNAs with unrelated primary sequence but with two or more hairpins displaying the RsmA/E binding motifs. The results support the hypothesis that the molecular mimicry of Rsm/Csr sRNAs is based on proper secondary structures that expose critical binding motifs irrespective of their overall sequence.
引用
收藏
页码:349 / 359
页数:11
相关论文
共 49 条
[21]   IDENTIFICATION AND LOCATION OF L-GLYCERATE, AN UNUSUAL ACYL SUBSTITUENT IN GELLAN GUM [J].
KUO, MS ;
MORT, AJ ;
DELL, A .
CARBOHYDRATE RESEARCH, 1986, 156 :173-187
[22]   Gac/Rsm signal transduction pathway of γ-proteobacteria:: from RNA recognition to regulation of social behaviour [J].
Lapouge, Karine ;
Schubert, Mario ;
Allain, Frederic H. -T. ;
Haas, Dieter .
MOLECULAR MICROBIOLOGY, 2008, 67 (02) :241-253
[23]   Mechanism of hcnA mRNA recognition in the Gac/Rsm signal transduction pathway of Pseudomonas fluorescens [J].
Lapouge, Karine ;
Sineva, Elena ;
Lindell, Magnus ;
Starke, Katja ;
Baker, Carol S. ;
Babitzke, Paul ;
Haas, Dieter .
MOLECULAR MICROBIOLOGY, 2007, 66 (02) :341-356
[24]   CsrA and three redundant small RNAs regulate quorum sensing in Vibrio cholerae [J].
Lenz, DH ;
Miller, MB ;
Zhu, J ;
Kulkarni, RV ;
Bassler, BL .
MOLECULAR MICROBIOLOGY, 2005, 58 (04) :1186-1202
[25]   The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli. [J].
Liu, MY ;
Gui, GJ ;
Wei, BD ;
Preston, JF ;
Oakford, L ;
Yuksel, U ;
Giedroc, DP ;
Romeo, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (28) :17502-17510
[26]   Molecular characterization of global regulatory RNA species that control pathogenicity factors in Erwinia amylovora and Erwinia herbicola pv. gypsophilae [J].
Ma, WL ;
Cui, Y ;
Liu, Y ;
Dumenyo, CK ;
Mukherjee, A ;
Chatterjee, AK .
JOURNAL OF BACTERIOLOGY, 2001, 183 (06) :1870-1880
[27]   Bacterial small RNA regulators [J].
Majdalani, N ;
Vanderpool, CK ;
Gottesman, S .
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 40 (02) :93-113
[28]   Salicylic acid biosynthetic genes expressed in Pseudomonas fluorescens strain P3 improve the induction of systemic resistance in tobacco against tobacco necrosis virus [J].
Maurhofer, M ;
Reimmann, C ;
Schmidli-Sacherer, P ;
Heeb, S ;
Haas, D ;
Défago, G .
PHYTOPATHOLOGY, 1998, 88 (07) :678-684
[29]  
Ongena M, 1999, PLANT PATHOL, V48, P66, DOI 10.1046/j.1365-3059.1999.00315.x
[30]   Posttranscriptional repression of GacS/GacA-controlled genes by the RNA-binding protein RsmE acting together with RsmA in Biocontrol strain Pseudomonas fluorescens CHA0 [J].
Reimmann, C ;
Valverde, C ;
Kay, E ;
Haas, D .
JOURNAL OF BACTERIOLOGY, 2005, 187 (01) :276-285