Induction of metaphase arrest in cleaving Xenopus embryos in by the protein kinase p90Rsk

被引：132
作者：
Gross, SD
Schwab, MS
Lewellyn, AL
Maller, JL [1 ]
机构：
[1] Univ Colorado, Sch Med, Howard Hughes Med Inst, Denver, CO 80262 USA
[2] Univ Colorado, Sch Med, Dept Pharmacol, Denver, CO 80262 USA
来源：
SCIENCE | 1999年 / 286卷 / 5443期
关键词：
D O I：
10.1126/science.286.5443.1365
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Before fertilization, vertebrate eggs are arrested in metaphase of meiosis II by cytostatic factor (CSF), an activity that requires activation of the mitogen-activated protein kinase (MAPK) pathway. To investigate whether CSF arrest is mediated by the protein kinase p90(Rsk), which is phosphorylated and activated by MAPK, a constitutively activated (CA) form of Rsk was expressed in Xenopus embryos. Expression of CA Rsk resulted in cleavage arrest, and cytological analysis showed that arrested blastomeres were in M phase with prominent spindles characteristic of meiotic metaphase. Thus, Rsk appears to be the mediator of MAPK-dependent CSF arrest in vertebrate unfertilized eggs.
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页码：1365 / 1367
页数：3
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