Seeing Atoms: Single-Particle Cryo-EM Breaks the Atomic Barrier

被引：8
|
作者：
Tan, Yong Zi [1 ]
Carragher, Bridget [2 ,3 ]
机构：
[1] Hosp Sick Children, Mol Med Program, Toronto, ON M5G 0A4, Canada
[2] New York Struct Biol Ctr, Natl Resource Automated Mol Microscopy, Simons Electron Microscopy Ctr, New York, NY 10027 USA
[3] Columbia Univ, Dept Biochem & Mol Biophys, 630 W 168th St, New York, NY 10032 USA
来源：
MOLECULAR CELL | 2020年 / 80卷 / 06期
基金：
加拿大健康研究院;
关键词：
D O I：
10.1016/j.molcel.2020.11.043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The goal of structural biology is to understand biological macromolecules in as much detail as possible. Depending on the resolution of the structure obtained, insights will range from understanding interactions at the level of proteins, domains, or atoms. The three mainstay structural biology techniques are X-ray crystallography, nuclear magnetic resonance (NMR) imaging, and cryogenic electron microscopy (cryo-EM). Cryo-EM has rapidly gained popularity in recent years due to a combination of hardware and software advances, leading to the so-called Resolution Revolution (Kuhlbrandt, 2014).
引用
收藏
页码：938 / 939
页数：2
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