Selection enhances protein evolvability by increasing mutational robustness and foldability

被引:53
作者
Zheng, Jia [1 ,2 ]
Guo, Ning [3 ]
Wagner, Andreas [1 ,2 ,4 ]
机构
[1] Univ Zurich, Dept Evolutionary Biol & Environm Studies, Zurich, Switzerland
[2] Swiss Inst Bioinformat, Quartier Sorge Batiment Genopode, Lausanne, Switzerland
[3] Zwirnereistr 11, Zurich, Switzerland
[4] Santa Fe Inst, Santa Fe, NM 87501 USA
基金
欧洲研究理事会; 瑞士国家科学基金会;
关键词
D O I
10.1126/science.abb5962
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Natural selection can promote or hinder a population's evolvability-the ability to evolve new and adaptive phenotypes-but the underlying mechanisms are poorly understood. To examine how the strength of selection affects evolvability, we subjected populations of yellow fluorescent protein to directed evolution under different selection regimes and then evolved them toward the new phenotype of green fluorescence. Populations under strong selection for the yellow phenotype evolved the green phenotype most rapidly. They did so by accumulating mutations that increase both robustness to mutations and foldability. Under weak selection, neofunctionalizing mutations rose to higher frequency at first, but more frequent deleterious mutations undermined their eventual success. Our experiments show how selection can enhance evolvability by enhancing robustness and create the conditions necessary for evolutionary success.
引用
收藏
页码:1183 / +
页数:9
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