Dual extraction of mRNA and lipids from a single biological sample

被引：11

作者：

Podechard, Normand ^{[1
,2
,3
]}

Ducheix, Simon ^{[1
]}

Polizzi, Arnaud ^{[1
]}

Lasserre, Frederic ^{[1
]}

Montagner, Alexandra ^{[1
,4
]}

Legagneux, Vincent ^{[2
,3
]}

Fouche, Edwin ^{[1
]}

Saez, Fabrice ^{[5
]}

Lobaccaro, Jean-Marc ^{[5
,6
]}

Lakhal, Laila ^{[1
]}

Ellero-Simatos, Sandrine ^{[1
]}

Martin, Pascal. G. ^{[1
]}

Loiseau, Nicolas ^{[1
]}

Bertrand-Michel, Justine ^{[4
,7
]}

Guillou, Herve ^{[1
]}

机构：

[1] Univ Toulouse, INRA, ToxAlim, UMR1331, Toulouse, France

[2] INSERM, UFR Sci Pharmaceut & Biol, UMR 1085, Irset, F-35043 Rennes, France

[3] Univ Rennes 1, CNRS 3480, UMS, Biosit SFR, F-35043 Rennes, France

[4] INSERM, Inst Metab & Cardiovasc Dis, UMR1048, Toulouse, France

[5] Univ Clermont Auvergne, CNRS, INSERM, GReD,UMR 6293,U1103, 28 Pl Henri Dunant,BP38, F-63001 Clermont Ferrand, France

[6] Ctr Rech Nutr Humaine Auvergne, 58 Blvd Montalembert, F-63009 Clermont Ferrand, France

[7] INSERM, Inst Metab & Cardiovasc Dis, UMR1048, Metatoul Lipid Facil,MetaboHUB, Toulouse, France

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

LIVER X RECEPTOR; HEPATIC STEATOSIS; GENE-EXPRESSION; LIPOGENESIS; SIGNATURE; TARGET; ALPHA; LXR;

D O I：

10.1038/s41598-018-25332-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The extraction of RNA and lipids from a large number of biological samples is time-consuming and costly with steps required for both transcriptomic and lipidomic approaches. Most protocols rely on independent extraction of nucleic acids and lipids from a single sample, thereby increasing the need for biological material and inducing variability in data analysis. We investigated whether it is possible to use a standard RNA extraction procedure to analyze not only RNA levels, but also lipids in a single liver sample. We show that the organic phase obtained when using standard reagents for RNA extraction can be used to analyze lipids, including neutral lipids and fatty acids, by gas chromatography. We applied this technique to an analysis of lipids and the associated gene expression pattern in mice with hepatic steatosis induced by pharmacological activation of nuclear receptor LXR.

引用

页数：11

共 28 条

[1] Circulating phospholipid profiling identifies portal contribution to NASH signature in obesity [J].