Human parenchymal and non-parenchymal liver cell isolation, culture and characterization

被引：33

作者：

Damm, Georg ^{[1
]}

Pfeiffer, Elisa ^{[1
]}

Burkhardt, Britta ^{[2
]}

Vermehren, Jan ^{[3
]}

Nuessler, Andreas K. ^{[2
]}

Weiss, Thomas S. ^{[3
]}

机构：

[1] Charite, Dept Gen Visceral & Transplant Surg, D-13353 Berlin, Germany

[2] Univ Tubingen, BG Trauma Ctr, Siegfried Weller Inst, BG Tubingen Siegfried Weller Inst, D-72076 Tubingen, Germany

[3] Univ Hosp Regensburg, Dept Pediat & Juvenile Med, D-93053 Regensburg, Germany

来源：

HEPATOLOGY INTERNATIONAL | 2013年 / 7卷 / 04期

关键词：

Human hepatocytes; Liver cell isolation; Parenchymal liver cells; Non-parenchymal liver cells; Liver cell culture; SINUSOIDAL ENDOTHELIAL-CELLS; HEPATIC STELLATE CELLS; KUPFFER CELLS; HUMAN HEPATOCYTES; INFILTRATING LYMPHOCYTES; RAT-LIVER; IN-VIVO; TRANSPLANTATION; EXPRESSION; LIPOCYTES;

D O I：

10.1007/s12072-013-9475-7

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Many reports describing parenchymal liver cell isolation have been published so far. However, recent evidence has clearly demonstrated that non-parenchymal liver cells play an important role in many pathophysiologies of the liver, such as drug-induced liver diseases, inflammation, and the development of liver fibrosis and cirrhosis. In this study, we present an overview of the current methods for isolating and characterizing parenchymal and non-parenchymal liver cells.

引用

页码：951 / 958

页数：8

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