The endocytosis of oxidized LDL via the activation of the angiotensin II type 1 receptor

被引：11

作者：

Takahashi, Toshimasa ^{[1
,2
]}

Huang, Yibin ^{[1
]}

Yamamoto, Koichi ^{[1
]}

Hamano, Go ^{[1
]}

Kakino, Akemi ^{[3
]}

Kang, Fei ^{[2
]}

Imaizumi, Yuki ^{[1
]}

Takeshita, Hikari ^{[1
]}

Nozato, Yoichi ^{[1
]}

Nozato, Satoko ^{[1
]}

Yokoyama, Serina ^{[1
]}

Nagasawa, Motonori ^{[1
]}

Kawai, Tatsuo ^{[1
]}

Takeda, Masao ^{[1
]}

Fujimoto, Taku ^{[1
]}

Hongyo, Kazuhiro ^{[1
]}

Nakagami, Futoshi ^{[1
]}

Akasaka, Hiroshi ^{[1
]}

Takami, Yoichi ^{[1
]}

Takeya, Yasushi ^{[1
]}

Sugimoto, Ken ^{[1
]}

Gaisano, Herbert Y. ^{[2
]}

Sawamura, Tatsuya ^{[3
]}

Rakugi, Hiromi ^{[1
]}

机构：

[1] Osaka Univ, Dept Geriatr & Gen Med, Grad Sch Med, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Univ Toronto, Dept Med, Toronto, ON M5S 1A8, Canada

[3] Shinshu Univ, Dept Mol Pathophysiol, Grad Sch Med, Matsumoto, Nagano 3908621, Japan

来源：

ISCIENCE | 2021年 / 24卷 / 02期

关键词：

LOW-DENSITY-LIPOPROTEIN; LECTIN-LIKE; PROTEIN; AT(1); MECHANISMS; LOX-1; ATHEROSCLEROSIS; MODULATION; BINDING; PHOSPHORYLATION;

D O I：

10.1016/j.isci.2021.102076

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Arrestin-dependent activation of a G-protein-coupled receptor (GPCR) triggers endocytotic internalization of the receptor complex. We analyzed the interaction between the pattern recognition receptor (PRR) lectin-like oxidized low-density lipoprotein (oxLDL) receptor (LOX-1) and the GPCR angiotensin II type 1 receptor (AT1) to report a hitherto unidentified mechanism whereby internalization of the GPCRmediates cellular endocytosis of the PRR ligand. Using geneticallymodified Chinese hamster ovary cells, we found that oxLDL activates Gai but not the G alpha q pathway of AT1 in the presence of LOX-1. Endocytosis of the oxLDL-LOX-1 complex through the AT1-beta-arrestin pathway was demonstrated by real-time imaging of the membrane dynamics of LOX-1 and visualization of endocytosis of oxLDL. Finally, this endocytotic pathway involving GPCR kinases (GRKs), beta-arrestin, and clathrin is relevant in accumulating oxLDL in human vascular endothelial cells. Together, our findings indicate that oxLDL activates selective G proteins and beta-arrestin-dependent internalization of AT1, whereby the oxLDL-LOX-1 complex undergoes endocytosis.

引用

页数：44

共 65 条

[1] Dual inhibition of β-adrenergic and angiotensin II receptors by a single antagonist -: A functional role for receptor-receptor interaction in vivo
Barki-Harrington, L
Luttrell, LM
Rockman, HA
[J]. CIRCULATION, 2003, 108 (13) : 1611 - 1618
[2] A new inhibitor of the β-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling
Beautrait, Alexandre
Paradis, Justine S.
Zimmerman, Brandon
Giubilaro, Jenna
Nikolajev, Ljiljana
Armando, Sylvain
Kobayashi, Hiroyuki
Yamani, Lama
Namkung, Yoon
Heydenreich, Franziska M.
Khoury, Etienne
Audet, Martin
Roux, Philippe P.
Veprintsev, Dmitry B.
Laporte, Stephane A.
Bouvier, Michel
[J]. NATURE COMMUNICATIONS, 2017, 8
[3] Bellot M, 2015, NAT CHEM BIOL, V11, P271, DOI [10.1038/NCHEMBIO.1766, 10.1038/nchembio.1766]
[4] Berk BC, 1999, J AM SOC NEPHROL, V10, pS62
[5] Mechanisms underlying adverse effects of HDL on eNOS-activating pathways in patients with coronary artery disease
Besler, Christian
Heinrich, Kathrin
Rohrer, Lucia
Doerries, Carola
Riwanto, Meliana
Shih, Diana M.
Chroni, Angeliki
Yonekawa, Keiko
Stein, Sokrates
Schaefer, Nicola
Mueller, Maja
Akhmedov, Alexander
Daniil, Georgios
Manes, Costantina
Templin, Christian
Wyss, Christophe
Maier, Willibald
Tanner, Felix C.
Matter, Christian M.
Corti, Roberto
Furlong, Clement
Lusis, Aldons J.
von Eckardstein, Arnold
Fogelman, Alan M.
Luescher, Thomas F.
Landmesser, Ulf
[J]. JOURNAL OF CLINICAL INVESTIGATION, 2011, 121 (07) : 2693 - 2708
[6] Vascular inflammation and the renin-angiotensin system
Brasier, AR
Recinos, A
Eledrisi, MS
[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (08) : 1257 - 1266
[7] Determining the environment of the ligand binding pocket of the human angiotensin II type I (hAT1) receptor using the methionine proximity assay
Clément, M
Martin, SS
Beaulieu, ME
Chamberland, C
Lavigne, P
Leduc, R
Guillemette, G
Escher, E
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (29) : 27121 - 27129
[8] Hyperch olesterolemia stimulates angiotensin peptide synthesis and contributes to atherosclerosis through the AT1A receptor
Daugherty, A
Rateri, DL
Lu, H
Inagami, T
Cassis, LA
[J]. CIRCULATION, 2004, 110 (25) : 3849 - 3857
[9] Characterization of Angiotensin II Molecular Determinants Involved in AT1 Receptor Functional Selectivity
Domazet, Ivana
Holleran, Brian J.
Richard, Alexandra
Vandenberghe, Camille
Lavigne, Pierre
Escher, Emanuel
Leduc, Richard
Guillemette, Gaetan
[J]. MOLECULAR PHARMACOLOGY, 2015, 87 (06) : 982 - 995
[10] Induction of Lysosomal Biogenesis in Atherosclerotic Macrophages Can Rescue Lipid-Induced Lysosomal Dysfunction and Downstream Sequelae
Emanuel, Roy
Sergin, Ismail
Bhattacharya, Somashubhra
Turner, Jaleisa N.
Epelman, Slava
Settembre, Carmine
Diwan, Abhinav
Ballabio, Andrea
Razani, Babak
[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2014, 34 (09) : 1942 - 1952

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