Vertical flow assays based on core-shell SERS nanotags for multiplex prostate cancer biomarker detection

被引:68
作者
Chen, Ruipeng [1 ,2 ,3 ]
Liu, Bing [1 ,2 ,3 ]
Ni, Haibin [1 ,2 ]
Chang, Ning [1 ,2 ]
Luan, Chengxin [4 ]
Ge, Qinyu [1 ,2 ]
Dong, Jian [1 ,2 ]
Zhao, Xiangwei [1 ,2 ,3 ]
机构
[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
[2] Southeast Univ, Natl Demonstrat Ctr Expt Biomed Engn Educ, Nanjing 210096, Jiangsu, Peoples R China
[3] Southeast Univ, Minist Educ, Key Lab Environm Med Engn, Nanjing 210096, Jiangsu, Peoples R China
[4] Southeast Univ, Sch Med, Zhongda Hosp, Dept Hematol & Oncol,Key Dept Jiangsu Med, Nanjing 210009, Jiangsu, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
ANTIGEN PSA; MICROARRAY ASSAY; TUMOR-MARKERS; GOLD; IMMUNOASSAY; NANOPARTICLES; IMMUNOSENSOR; POINT; GRAPHENE; AMPLIFICATION;
D O I
10.1039/c9an00733d
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Rapid, simultaneous, and sensitive quantification of multiplex prostate biomarkers plays an important role in early diagnosis, especially for obese men and patients. Herein, a surface-enhanced Raman scattering (SERS)-based vertical flow assay (VFA) is presented for simultaneous detection of multiplex prostate cancer biomarkers, such as prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), and alpha-fetoprotein (AFP) on a single test spot. In practice, Raman dyes (RDs) encoded core-shell SERS nanotags instead of conventional gold colloids used in the colorimetric assay are employed in the sensing membrane of SERS based VFAs for multiplex protein detection. Because of the enhanced Raman signal of the core-shell nanostructure and the high surface area to volume ratio (SVR) of the porous sensing membrane, this proposed biosensor shows a wide linear dynamic range (LDR) with detection limits of 0.37, 0.43, and 0.26 pg mL(-1) for PSA, CEA, and AFP, respectively, suggesting that this approach can be a good candidate in point of care testing (POCT) for rapid and sensitive biomarker detection and has a huge potential in multiplex analysis and cancer diagnosis.
引用
收藏
页码:4051 / 4059
页数:9
相关论文
共 54 条
[1]   Power-free chip enzyme immunoassay for detection of prostate specific antigen (PSA) in serum [J].
Ahmed, Heba Adel ;
Azzazy, Hassan M. E. .
BIOSENSORS & BIOELECTRONICS, 2013, 49 :478-484
[2]  
Albuquerque C. D. L. D., 2018, ANAL CHEM, V90, P1248, DOI DOI 10.1021/ACS.ANALCHEM.7B03968
[3]   Biosensing with plasmonic nanosensors [J].
Anker, Jeffrey N. ;
Hall, W. Paige ;
Lyandres, Olga ;
Shah, Nilam C. ;
Zhao, Jing ;
Van Duyne, Richard P. .
NATURE MATERIALS, 2008, 7 (06) :442-453
[4]   Vertical-flow paper SERS system for therapeutic drug monitoring of flucytosine in serum [J].
Berger, Adam G. ;
Restaino, Stephen M. ;
White, Ian M. .
ANALYTICA CHIMICA ACTA, 2017, 949 :59-66
[5]   Theoretical study of the optical absorption behavior of Au/Ag core-shell nanoparticles [J].
Bruzzone, S ;
Arrighini, GP ;
Guidotti, C .
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2003, 23 (6-8) :965-970
[6]   PSA Detection with Femtomolar Sensitivity and a Broad Dynamic Range Using SERS Nanoprobes and an Area-Scanning Method [J].
Chang, Hyejin ;
Kang, Homan ;
Ko, Eunbyeol ;
Jun, Bong-Hyun ;
Lee, Ho-Young ;
Lee, Yoon-Sik ;
Jeang, Dae Hong .
ACS SENSORS, 2016, 1 (06) :645-649
[7]   Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites [J].
Chen, Xia ;
Jia, Xinle ;
Han, Jingman ;
Ma, Jie ;
Ma, Zhanfang .
BIOSENSORS & BIOELECTRONICS, 2013, 50 :356-361
[8]   Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer [J].
Cheng, Ziyi ;
Choi, Namhyun ;
Wang, Rui ;
Lee, Sangyeop ;
Moon, Kyung Chul ;
Yoon, Soo-Young ;
Chen, Lingxin ;
Choo, Jaebum .
ACS NANO, 2017, 11 (05) :4926-4933
[9]   Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept [J].
Chinnasamy, Thiruppathiraja ;
Segerink, Loes I. ;
Nystrand, Mats ;
Gantelius, Jesper ;
Svahn, Helene Andersson .
CLINICAL CHEMISTRY, 2014, 60 (09) :1209-1216
[10]   Development of a SERS-Based Rapid Vertical Flow Assay for Point-of Care Diagnostics [J].
Clarke, O. J. R. ;
Goodall, B. L. ;
Hui, H. P. ;
Vats, N. ;
Brosseau, C. L. .
ANALYTICAL CHEMISTRY, 2017, 89 (03) :1405-1410