First detection and full genome sequence of porcine circovirus type 3 in Russia

被引：72
作者：
Yuzhakov, Anton G. [1 ]
Raev, Sergei A. [1 ]
Alekseev, Konstantin P. [1 ]
Grebennikova, Tatiana V. [1 ]
Verkhovsky, Oleg A. [1 ]
Zaberezhny, Alexei D. [2 ]
Aliper, Taras I. [2 ]
机构：
[1] Res Inst Human & Anim Dis, Lab Virol Diagnost & Prevent, Moscow, Russia
[2] YR Kovalenko All Russian Res Inst Expt Vet Med, Moscow, Russia
来源：
VIRUS GENES | 2018年 / 54卷 / 04期
关键词：
PCV3; Porcine circovirus; Complete genome; Swine; Sequencing; GENETIC-CHARACTERIZATION;
D O I：
10.1007/s11262-018-1582-z
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Porcine circovirus type 3 (PCV3) was firstly detected in 2016 in USA. Later PCV3 was discovered in Asia, Europe, and South America. The present investigation demonstrates for the first time the circulation of PCV3 among pigs in Russia. The viruses were detected at two geographically distant unrelated commercial farms with records of reproductive failure (abortions, stillbirth), porcine dermatitis, and nephropathy syndrome (PDNS). The two farms were located in the region of Smolensk (western part of Russia) and the region of Tyumen (West Siberia, Russia). We investigated samples collected from pigs of different ages. We performed PCR for the PCV3 DNA detection. The DNA of PCV3 was detected in serum, kidney, heart, spleen, pleural effusion, and peritoneal cavity fluid samples. Two viral genomes were sequenced and the corresponding strains were named PCV3-RU/SM17 (the strain from Smolensk region) and PCV3-RU/TY17 (the strain from Tyumen region). The full genome sequences of both strains were 2000 nucleotides in length and contained at least two ORFs, encoding the Cap and Rep proteins. Full sequence alignment revealed a 99.3% identity between the PCV3-RU/SM17 and PCV3-RU/TY17 strains. Molecular analysis showed that the two strains from Russia are highly homologous to viruses identified in other countries, with a 98.5-99.6% homology for PCV3-RU/TY17 and 97.9-99.0 for PCV3-RU/SM17. The PCV3-RU/SM17 and PCV3-RU/TY17 strains were found to form a monophyletic group in a phylogenetic tree based on PCV3 complete genome sequences.
引用
收藏
页码：608 / 611
页数：4
相关论文
共 18 条
[1]   Porcine circovirus type 3 in the UK [J].
Collins, P. J. ;
McKillen, John ;
Allan, Gordon .
VETERINARY RECORD, 2017, 181 (22)
[2]   Detection and genetic characterization of Porcine circovirus type 3 in Italy [J].
Faccini, S. ;
Barbieri, I. ;
Gilioli, A. ;
Sala, G. ;
Gibelli, L. R. ;
Moreno, A. ;
Sacchi, C. ;
Rosignoli, C. ;
Franzini, G. ;
Nigrelli, A. .
TRANSBOUNDARY AND EMERGING DISEASES, 2017, 64 (06) :1661-1664
[3]  
Fan SX, 2017, GENOME ANNOUNCEMENTS, V5, DOI [10.1128/genomeA.00100-17, 10.1128/genomea.00100-17]
[4]   Full-genome sequencing of porcine circovirus 3 field strains from Denmark, Italy and Spain demonstrates a high within-Europe genetic heterogeneity [J].
Franzo, G. ;
Legnardi, M. ;
Hjulsager, C. K. ;
Klaumann, F. ;
Larsen, L. E. ;
Segales, J. ;
Drigo, M. .
TRANSBOUNDARY AND EMERGING DISEASES, 2018, 65 (03) :602-606
[5]   Full genome characterization of porcine circovirus type 3 isolates reveals the existence of two distinct groups of virus strains [J].
Fux, Robert ;
Soeckler, Christina ;
Link, Ellen Kathrin ;
Renken, Christine ;
Krejci, Roman ;
Sutter, Gerd ;
Ritzmann, Mathias ;
Eddicks, Matthias .
VIROLOGY JOURNAL, 2018, 15
[6]   Porcine circovirus type 3 (PCV3) infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex (PRDC) [J].
Kedkovid, Roongtham ;
Woonwong, Yonlayong ;
Arunorat, Jirapat ;
Sirisereewan, Chaitawat ;
Sangpratum, Nattaphong ;
Lumyai, Mongkol ;
Kesdangsakonwut, Sawang ;
Teankum, Komkrich ;
Jittimanee, Suphattra ;
Thanawongnuwech, Roongroje .
VETERINARY MICROBIOLOGY, 2018, 215 :71-76
[7]  
King A.M., 2012, Ninth report of the International Committee on Taxonomy of Viruses
[8]   Identification and genetic characterization of porcine circovirus type 3 in China [J].
Ku, X. ;
Chen, F. ;
Li, P. ;
Wang, Y. ;
Yu, X. ;
Fan, S. ;
Qian, P. ;
Wu, M. ;
He, Q. .
TRANSBOUNDARY AND EMERGING DISEASES, 2017, 64 (03) :703-708
[9]  
Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msw054, 10.1093/molbev/msv279]
[10]   Prevalence of novel porcine circovirus 3 in Korean pig populations [J].
Kwon, Taeyong ;
Yoo, Sung J. ;
Park, Choi-Kyu ;
Lyoo, Young S. .
VETERINARY MICROBIOLOGY, 2017, 207 :178-180
12