Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater

被引：12

作者：

Elahi, Amina ^{[1
]}

Rehman, Abdul ^{[1
]}

机构：

[1] Univ Punjab, Microbiol & Mol Genet, Lahore, Pakistan

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2017年 / 60卷

关键词：

Trichosporon asahii; Rhodotorula mucilaginosa; wastewater; resistance; bioaccumulation; CR(VI) REDUCTION; CHROMATE-RESISTANT; HEXAVALENT CHROMIUM; STRAIN; YEAST; GLUTATHIONE; CELL; BIOREMEDIATION; ACCUMULATION; MEMBRANE;

D O I：

10.1590/1678-4324-2017160394

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 +/- 10.01) and GSSG (11.24 +/- 0.96) was observed under metal stress in T. asahii as compared to R. mucilaginosa GSH (18.95 +/- 3.19) and GSSG (3.7 +/- 2.74) mM g-1 8 level. Statistical analysis revealed significantly higher GSH/GSSG ratio in both strains. NPSH (29.84 +/- 0.54) level in T. asahii was much higher than in R. mucilaginosa (6.05 +/- 0.24). Chromate reductase (ChR) was assayed and its activity was optimum at 50 degrees C (pH 6) in T. asahii while R. mucilaginosa showed higher activity at 30 degrees C (pH 7). Activity of both ChRs was enhanced in the presence of Mg, Na, Co and Ca but strongly inhibited by Hg cations. Cr(VI) uptake capabilities were ranged between 43-97% in R. mucilaginosa and 35-88% in T. asahii. One dimensional electrophoresis revealed enriched bands of cysteine rich metallothioneins suggesting some differential proteins could be overexpressed under Cr(VI) stress.

引用

页数：14

共 52 条

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