Inhibitory effects of lysophosphatidic acid receptor-5 on cellular functions of sarcoma cells

被引：15

作者：

Araki, Mutsumi ^{[1
]}

Kitayoshi, Misaho ^{[1
]}

Dong, Yan ^{[1
]}

Hirane, Miku ^{[1
]}

Ozaki, Shuhei ^{[1
]}

Mori, Shiori ^{[1
]}

Fukushima, Nobuyuki ^{[2
]}

Honoki, Kanya ^{[3
]}

Tsujiuchi, Toshifumi ^{[1
]}

机构：

[1] Kinki Univ, Fac Sci & Engn, Dept Life Sci, Div Canc Biol & Bioinformat, Higashiosaka, Osaka 5778502, Japan

[2] Kinki Univ, Fac Sci & Engn, Dept Life Sci, Div Mol Neurobiol, Higashiosaka, Osaka 5778502, Japan

[3] Nara Med Univ, Dept Orthoped Surg, Kashihara, Nara 634, Japan

来源：

GROWTH FACTORS | 2014年 / 32卷 / 3-4期

基金：

日本学术振兴会;

关键词：

Lysophosphatidic acid; LPA receptor-5; malignant fibrous histiocytoma; osteosarcoma; tumor progression; OVARIAN-CANCER CELLS; TUMOR-CELLS; EXPRESSION; MIGRATION; INDUCTION; GENE; PROLIFERATION; BIOLOGY; LPA(3); ROLES;

D O I：

10.3109/08977194.2014.911294

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Lysophosphatidic acid (LPA) is a bioactive lipid that interacts with G protein-coupled LPA receptors (LPA receptor-1 (LPA(1)) to LPA(6)). Here, we investigated the effects of LPA signaling via LPA(5) on cellular functions of sarcoma cells by generating Lpar5 overexpressing and Lpar5 knockdown cells from rat osteosarcoma and malignant fibrous histiocytoma cells, respectively. The cell motility activity of Lpar5 overexpressing cells was significantly lower, while Lpar5 knockdown cells showed high cell motility, compared with respective controls. Gelatin zymography showed that LPA(5) suppressed the activation of matrix metalloproteinase-2. LPA(5) also inhibited the cell motility activity of endothelial cells, correlating with the expression levels of vascular endothelial growth factor genes. These results suggest that LPA signaling via LPA(5) negatively regulates the cellular functions of rat sarcoma cells.

引用

页码：117 / 122

页数：6

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