A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells

被引：28

作者：

Burger, Nina ^{[1
]}

Biswas, Abin ^{[1
,2
]}

Barzan, Daniel ^{[3
,4
]}

Kirchner, Anne ^{[1
]}

Hosser, Hiltraud ^{[5
]}

Hausmann, Michael ^{[2
]}

Hildenbrand, Georg ^{[1
,2
]}

Herskind, Carsten ^{[1
]}

Wenz, Frederik ^{[1
]}

Veldwijk, Marlon R. ^{[1
]}

机构：

[1] Heidelberg Univ, Univ Med Mannheim, Med Fac Mannheim, Dept Radiat Oncol, D-68135 Mannheim, Germany

[2] Heidelberg Univ, Kirchhoff Inst Phys, Heidelberg, Germany

[3] Heidelberg Univ, Cellnetworks Cluster, Heidelberg, Germany

[4] Heidelberg Univ, Inst Phys Chem, Heidelberg, Germany

[5] Heidelberg Univ, Univ Med Mannheim, Med Fac Mannheim, Ctr Biomed & Med Technol Mannheim CBTM,Dept Anat, D-68135 Mannheim, Germany

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2014年 / 10卷 / 06期

关键词：

Gold nanoparticles; Auger electrons; Transfection; Radiosensitization; Uptake; Size dependency; DOSE ENHANCEMENT; X-RAYS; TOTAL-BODY; TUMOR; ACCUMULATION; IRRADIATION; MECHANISM; DAMAGE; SIZE;

D O I：

10.1016/j.nano.2014.03.011

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Gold nanoparticles (GNP) enhance the absorbance of photons thereby increasing emission of Auger-/photoelectrons in the nm-mu m range. Yet, a major disadvantage is their diameter-dependent cellular uptake with an optimum of similar to 50 nm which may not offer optimal radiosensitization. A method was developed to enhance the uptake of small GNP. GNP (10 nm) were linked to DNA and transferred into HeLa cells by transient transfection (GNP-DT). Treatment of cells with GNP-DT resulted in a strong perinuclear focal accumulation, whereas this was dimmer and sparser for GNP-T (lacking DNA) and close to background levels in GNP-treated cells. Only GNP-DT showed a significant radiosensitizing effect (p = 0.005) on clonogenic survival using clinically relevant megavolt x-rays. Our novel method markedly increases the uptake/retention and alters the localization of small GNP in cells compared to unmodified GNP. This work finally enables studying the radiosensitizing effects of differentially sized GNP.

引用

页码：1365 / 1373

页数：9

共 31 条

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