The use of the replicating pDblet plasmid as a cloning vector with enhanced stability in Kluyveromyces marxianus

被引：7
作者：
de Souza, CG
de Morais, MA
机构：
[1] Univ Fed Pernambuco, Setor Biol Mol LIKA, BR-50732901 Recife, PE, Brazil
[2] Univ Fed Pernambuco, Dept Genet, BR-50732901 Recife, PE, Brazil
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 01期
关键词：
Kluyveromyces marxianus; plasmid stability; recombination; transformation efficiency;
D O I：
10.1023/A:1005624913974
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The replicating plasmid, pDblet, transformed the budding yeast Kluyveromyces marxianus to an efficiency of 10(4) transformant mu g(-1) DNA. Transformed cells showed 1% of segregation rate without affecting their growth rate of 0.69 h(-1) and glucose consumption. These results were similar or better than the commonly used pE1 plasmid and suggests that pDblet can be used for cloning genes in K. marxianus.
引用
收藏
页码：43 / 45
页数：3
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