Identification and localization of the bilitranslocase homologue in white grape berries (Vitis vinifera L.) during ripening

被引:9
作者
Bertolini, Alberto [1 ]
Peresson, Carlo [1 ]
Petrussa, Elisa [1 ]
Braidot, Enrico [1 ]
Passamonti, Sabina [2 ]
Macrì, Francesco [1 ]
Vianello, Angelo [1 ]
机构
[1] Univ Udine, Dipartimento Biol & Protez Piante, Sez Biol Vegetale, I-33100 Udine, Italy
[2] Univ Trieste, Dipartimento Sci Vita, I-34127 Trieste, Italy
关键词
Bilitranslocase; flavonoid transport; immunohistochemistry; seed; Vitis vinifera L; white berry cultivar; ANTHOCYANIN BIOSYNTHESIS; SULFOBROMOPHTHALEIN TRANSPORT; TRANSCRIPTIONAL REGULATION; CELLULAR-LOCALIZATION; MEMBRANE-TRANSPORT; INHIBITION; FLAVONOIDS; TANNINS; PATHWAY; SKINS;
D O I
10.1093/jxb/erp225
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A homologue of the mammalian bilirubin transporter bilitranslocase (BTL) (TCDB 2.A.65.1.1), able to perform an apparent secondary active transport of flavonoids, has previously been found in carnation petals and red grape berries. In the present work, a BTL homologue was also shown in white berries from Vitis vinifera L. cv. Tocai/Friulano, using anti-sequence antibodies specific for rat liver BTL. This transporter, similarly to what found in red grape, was localized in the first layers of the epidermal tissue and in the vascular bundle cells of the mesocarp. In addition, a strong immunochemical reaction was detected in the placental tissue and particularly in peripheral integuments of the seed. The protein was expressed during the last maturation stages in both skin and pulp tissues and exhibited an apparent molecular mass of c. 31 kDa. Furthermore, the transport activity of such a carrier, measured as bromosulphophthalein (BSP) uptake, was detected in berry pulp microsomes, where it was inhibited by specific anti-BTL antibodies. The BTL homologue activity exhibited higher values, for both K-m and V-max, than those found in the red cultivar. Moreover, two non-pigmented flavonoids, such as quercetin (a flavonol) and eriodictyol (a flavanone), inhibited the uptake of BSP in an uncompetitive manner. Such results strengthen the hypothesis that this BTL homologue acts as a carrier involved also in the membrane transport of colourless flavonoids and demonstrate the presence of such a carrier in different organs and tissues.
引用
收藏
页码:3861 / 3871
页数:11
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