Exploring inhibition mechanism and nature of lipase by Ligupurpuroside A extracted from Ku-Ding tea

被引:9
作者
Fan, Yu [1 ]
Xu, Yang [1 ,2 ]
Han, Qing-Guo [1 ]
Shen, Liang-Liang [1 ]
Xu, Hong [1 ,3 ]
Wu, Zhi-Bing [1 ]
Xu, Xu [1 ]
Ying, Ming [1 ]
He, Zhen-Dan [4 ]
Hu, Zhang-Li [1 ]
机构
[1] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Bioresources & Ecol, Shenzhen Key Lab Microbial Genet Engn, Shenzhen 518055, Peoples R China
[2] Shenzhen High Sch, Shenzhen 518001, Peoples R China
[3] Hangzhou Dianzi Univ, Minist Educ, Key Lab RF Circuits & Syst, Hangzhou 310018, Zhejiang, Peoples R China
[4] Shenzhen Univ, Sch Med, Shenzhen 518060, Peoples R China
基金
中国国家自然科学基金;
关键词
Ligupurpuroside A; Lipase activity; Inhibitor; Binding mechanism; Interaction; BOVINE SERUM-ALBUMIN; SPECTROSCOPIC METHODS; PHENYLETHANOID GLYCOSIDES; LIGUSTRUM-PURPURASCENS; MOLECULAR DOCKING; BINDING; PEPSIN; FLUORESCENCE; OBESITY; QUERCETIN;
D O I
10.1007/s00044-018-2194-9
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Enzyme-activity essay demonstrated that Ligupurpuroside A acts as a natural inhibitor of lipase in a competitive manner. The inhibition mechanism and nature of lipase by Ligupurpuroside A were investigated by fluorescence spectra, UV-Vis absorption spectra, circular dichroism (CD) spectra and molecular docking methods. Fluorescence experiments indicated that Ligupurpuroside A can quench the intrinsic fluorescence of lipase through a static quenching procedure. Thermodynamic analysis suggested that hydrophobic interaction is the main force between lipase and Ligupurpuroside A. Fluorescence resonance energy transfer experiment showed that an energy transfer from lipase to Ligupurpuroside A occurs with great possibility, confirming the presence of static quenching mechanism of lipase fluorescence by Ligupurpuroside A. Interestingly, conformation of lipase remained almost the same after bound to Ligupurpuroside A based on CD spectral experiments. All these experimental results were validated by the protein-ligand docking studies which further showed that Ligupurpuroside A could bind to the amino acid residues of the hydrophobic cavity on catalytic sites of lipase. This study should be helpful for the design of diet drug and the full use of Ligupurpuroside A in food industry.
引用
收藏
页码:1822 / 1833
页数:12
相关论文
共 46 条
[1]  
[Anonymous], 1965, MODERN QUANTUM CHEM
[2]   Investigation of three flavonoids binding to bovine serum albumin using molecular fluorescence technique [J].
Bi, Shuyun ;
Yan, Lili ;
Pang, Bo ;
Wang, Yu .
JOURNAL OF LUMINESCENCE, 2012, 132 (01) :132-140
[3]   Spectroscopic and voltammetric characterizations of the interaction of two local anesthetics with bovine serum albumin [J].
Bi, Shuyun ;
Yan, Lili ;
Wang, Binbin ;
Bian, Jiangyu ;
Sun, Yantao .
JOURNAL OF LUMINESCENCE, 2011, 131 (05) :866-873
[4]   Pancreatic lipase inhibitors from natural sources: unexplored potential [J].
Birari, Rahul B. ;
Bhutani, Kamiesh K. .
DRUG DISCOVERY TODAY, 2007, 12 (19-20) :879-889
[5]   Interaction Between Trans-resveratrol and Serum Albumin in Aqueous Solution [J].
Cao, Shuhong ;
Wang, Dandan ;
Tan, Xiaoyan ;
Chen, Jingwen .
JOURNAL OF SOLUTION CHEMISTRY, 2009, 38 (09) :1193-1202
[6]   Recent findings of green tea extract AR25 (Exolise) and its activity for the treatment of obesity [J].
Chantre, P ;
Lairon, D .
PHYTOMEDICINE, 2002, 9 (01) :3-8
[7]   Polyphenol/peptide binding and precipitation [J].
Charlton, AJ ;
Baxter, NJ ;
Khan, ML ;
Moir, AJG ;
Haslam, E ;
Davies, AP ;
Williamson, MP .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (06) :1593-1601
[8]   The obesity pipeline: current strategies in the development of anti-obesity drugs [J].
Cooke, Dunstan ;
Bloom, Steve .
NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (11) :919-931
[9]   Characterization of 6-mercaptopurine binding to bovine serum albumin and its displacement from the binding sites by quercetin and rutin [J].
Ehteshami, Mehdi ;
Rasoulzadeh, Farzaneh ;
Mahboob, Soltanali ;
Rashidi, Mohammad-Reza .
JOURNAL OF LUMINESCENCE, 2013, 135 :164-169
[10]   Study on the mechanism of the interaction between acteoside and pepsin using spectroscopic techniques [J].
Fang, Yifeng ;
Xu, Hong ;
Shen, Liangliang ;
Huang, Fengwen ;
Yibulayin, Shadaiti ;
Huang, Songyang ;
Tian, Shengli ;
Hu, Zhangli ;
He, Zhendan ;
Li, Fangrong ;
Li, Yinong ;
Zhou, Kai .
LUMINESCENCE, 2015, 30 (06) :859-866